Sequences from confirmation amplicon


Confirmation sequences for line 057F02 and hit At2g37760 are shown.
Line ID 057F02
Locus At2g37760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15833686
Primers used for PCR 01qa - 8474, show primer details
   
Sequence Name 85-K115459-0022-057-F02C-01qa
GenBank Accession LN514578 [GenBank]
Sequencing primer gene specific primer, 01qa: 5'-TTTTTGCGGTATGTAGAAAATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K115459-0022-057-F02C-01qa
GTTGTAACTANTTGAAAACATTTTATTGTTTGAAGAATGCAGAATAACAACATTTGCCAA
ACATCATGAAATCCCAAGCATGTGTTTAGTGTTTTCTCATAGAAAGATTAAATATAAAAA
GATAACATATTTATTTCAATGTCCTTAATGGTTTATGGTTAAACAAGTAGAAAGGAGGGA
CATTATCTCAAATTTCACCGTCCCACAATTCCTCAATGGTCTTATAGAAGCCGTGTGTCT
CATGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCACAATTATT
   
Sequence Name 36-K115458-0022-057-F02C-8409
GenBank Accession LN502347 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K115458-0022-057-F02C-8409
CATTTCATTGAATATATTCATGAGACACACGGCTTCTATAAGACCATTGAGGAATTGTGG
GACGGTGAAATTTGAGATAATGTCCCTCCTTTCTACTTGTTTAACCATAAACCATTAAGG
ACATTGAAATAAATATGTTATCTTTTTATATTTAATCTTTCTATGAGAAAACACTAAACA
CATGCTTGGGATTTCATGATGTTTGGCAAATGTTGTTATTCTGCATTCTTCAAACAATAA
AATGTTTTCAACTAGTTACAACTTCAACATGACATAGTCGATAGAACCTAAGACTATTTC
CTTAGTTCGCGTCGGGAATTTTCTACTACNCGCAAAA

Last Updated on 06.12.2018 16:11