Sequences from confirmation amplicon


Confirmation sequences for line 058C11 and hit At5g57100 are shown.
Line ID 058C11
Locus At5g57100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23106581
Primers used for PCR 03op - 8474, show primer details
   
Sequence Name 29-K114909-0022-058-C11C-03op
GenBank Accession LN499411 [GenBank]
Sequencing primer gene specific primer, 03op: 5'-CGTTTGTTTTCAGTCACACTCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114909-0022-058-C11C-03op
GAAAACGACGCCAATCACTCTGTTGTTTCTTGTATCGATGATTCCGTTCTTGGATCCGCC
CGGTGCTCTATCGTTCAATTGGAGCTTAACCAACACATCTGCCATTCTTGTTTCTGCTCT
TCTCGGATTCTTTCTTCAATGGTCAGGAGCCTTAGCTCTAGGGTGAGTAAAAGTTTCTTA
TACATTAGTGTTGTCGTTACATTTTTGGATTTAAAGGTTGCGTAGCTGAGTGGTTCCTTA
AGTCCAAATTTTGCTCCATATATTGCAGAGCAACTTCTGCAATTACGCATGTGGTGCTAG
GACAGTTCAAGACTTGTGTCCTCCTCCTTGGAAACTACTACATTTTCGGGTCGAATAGTG
GTTTCATTAGCGTTGGCGGTGCGTTTGTGGCGATTATGGGAACTTCGTTATATACATACC
TTAACACGAGAGGTCAATCTCTTAAAACTTCGTCTTCTTCTTCTGCTCTCTCGGAGAAGA
AGTCAAGATTCAGTGATCTTAAGGATGATGACNAGAATCTTGAACCTTACATGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATT
   
Sequence Name 05-K114908-0022-058-C11C-8409
GenBank Accession LN488464 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114908-0022-058-C11C-8409
CATGTAAGGTTCAAGATTCTTGTCATCATCCTTAAGATCACTGAATCTTGACTTCTTCTC
CGAGAGAGCAGAAGAAGAAGACGAAGTTTTAAGAGATTGACCTCTCGTGTTAAGGTATGT
ATATAACGAAGTTCCCATAATCGCCACAAACGCACCGCCAACGCTAATGAAACCACTATT
CGACCCGAAAATGTAGTAGTTTCCAAGGAGGAGGACACAAGTCTTGAACTGTCCTAGCAC
CACATGCGTAATTGCAGAAGTTGCTCTGCAATATATGGAGCAAAATTTGGACTTAAGGAA
CCACTCAGCTACGCAACCTTTAAATCCAAAATGTAACGACAACACTAATGTATAAGAAAC
TTTTACTCACCCTAGAGCTAAGGCTCCTGACCATTGAAGAAAGAATCCGAGAAGAGCAGA
AACAAGAATGGCAGATGTGTTGGTTAAGCTCCAATTGAACGATAGAGCACCGGGCGGATC
CNAGAACGGAATCATCGATACAAGAAACAACAGAGTGATTGGCGTCGTTTTCCACATCAA
CCTGAATTAAAAAATAAAAATGAAGAGATGGTATTGAGAGATGTTGATACAGCTATATGA
ATGAGTGT

Last Updated on 06.12.2018 16:11