Sequences from confirmation amplicon


Confirmation sequences for line 060A04 and hit At1g66580 are shown.
Line ID 060A04
Locus At1g66580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24839216
Primers used for PCR BG62 - 8474, show primer details
   
Sequence Name 06-K104021-0022-060-A04C-BG62
GenBank Accession LN488785 [GenBank]
Sequencing primer gene specific primer, BG62: 5'-TAGAATGGAGCTTATGTTGGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K104021-0022-060-A04C-BG62
GTTTTACTTATGTGTGATTGCTTAGCCAATGTGGACTCTGAAGATGGTTTTCAGTTTTAG
ATATGAATGTCCTTGTTACTATTGTTACACAGTCACACAGAGTTTACAAAAGATCATTCA
TCATTGTTTATAAGAGAACAAATTGATAACATATATCTTTTTGATGTGGATGGATTTGTT
AACCCTCTAGAAGAACACCCAAATTTGGCTACAAAGTTGTCAACACATTGTTCTTCGTTC
TTCTTTGACTTTGACTAAAAATAAAAATAAACCTCGGCCTAAGAAAATAGCGTGTTCACA
TTGGATGATTAAGGCCTGGACCGAGTATCAAATAATATCTAAATGGGCCTAGCTAAGCAG
TTACGTAAGCTAAATTTAGGGTTTGTTTTTGCCGCACCTATNAAATAACAACACAGCTTC
CCATTTGTAATTTCTAGGGTTTTGCAAAACCAACACCGAAAATCCAACACGATGGGACGA
TTCANTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATG
TCC
   
Sequence Name 22-K104022-0022-060-A04C-8409
GenBank Accession LN496087 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K104022-0022-060-A04C-8409
TGAGCCATTTACAATTGAATCGTCCCATCGTGTTGGATCTTCGGTGTTGGTTTTGCAAAA
CCCTAGAAATTACAAATGGGAAGCTGTGTTGTTATTTTATAGGTGCGGCAAAAACAAACC
CTAAATTTAGCTTACGTAACTGCTTAGCTAGGCCCATTTAGATATTATTTGATACTCGGT
CCAGGCCTTAATCATCCAATGTGAACACGCTATTTTCTTAGGCCGAGGTTTATTTTTATT
TTTAGTCAAAGTCAAAGAAGAACGAAGAACAATGTGTTGACAACTTTGTAGCCAAATTTG
GGTGTTCTTCTAGAGGGTTAACAAATCCATCCACATCAAAAAGATATATGTTATCAATTT
GTTCTCTTATAAACAATGATGAATGATCTTTTGTAAACTCTGTGTGACTGTGTAACAATA
GTAACAAGGACATTCATATCTAAAACTGAAAACCATCTTCAGAGTCCACATTTGGCTAAG
CAATCACACATAAGTTAAAAACCATTTTGAAACAAAAACAAAAGCCAACATAAG

Last Updated on 06.12.2018 16:11