Sequences from confirmation amplicon


Confirmation sequences for line 061A12 and hit At1g06160 are shown.
Line ID 061A12
Locus At1g06160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1883502
Primers used for PCR KM58 - 8474, show primer details
   
Sequence Name 08-K101217-0022-061-A12C-8409
GenBank Accession LN489665 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K101217-0022-061-A12C-8409
CGGAATGAGCCTTTCATTCTCATCTTCCGGAGAGATTCTTCACGACATCCGCGGGGAAAT
TGAGTACTGCGAGGCTGCCTTTCAAAGCGAAAGCCGCCTGATCATAAGCGAGAGCCGCCG
CCTCCGCGGTGTCGAATGTCCCAAGCCACACTCTTATCCCTTTCCTCGTTGAGTCTCTTA
TCTCGGCCGCGAATTTCCCCCACGGCCGTTTCCTCACTCCTCTGTATGACTTCTCCTCCT
CCTCGGCCGCCACGGTGGCTTCTTTTCCTTCTTCTTTTATGATTGATGATTCAAATGCAA
AGAAGTC
   
Sequence Name 08-K101223-0022-061-A12C-KM58
GenBank Accession LN489666 [GenBank]
Sequencing primer gene specific primer, KM58: 5'-TGACTTCTTTGCATTTGAATCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K101223-0022-061-A12C-KM58
GAAGAGCCCCGTGGCGGCCGAGAGGAGGAGAAGTCATACGAGGAGTGAGGAAACGGCCGT
GGGGGAAATTCGCGGCCGAGATAAGAGACTCAACGAGGAAAGGGATAAGAGTGTGGCTTG
GGACATTCGACACCGCGGAGGCGGCGGCTCTCGCTTATGATCAGGCGGCTTTCGCTTTGA
AAGGCAGCCTCGCAGTACTCAATTTCCCCGCGGATGTCGTTGAAGAATCTCTCCGGAAGA
TGGAGAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCACACGGTTATA

Last Updated on 06.12.2018 16:11