Sequences from confirmation amplicon


Confirmation sequences for line 062D06 and hit At3g28070 are shown.
Line ID 062D06
Locus At3g28070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10448979
Primers used for PCR PC35 - 8474, show primer details
   
Sequence Name 38-K103731-0022-062-D06C-PC35
GenBank Accession LN502950 [GenBank]
Sequencing primer gene specific primer, PC35: 5'-GTGTTCGTATGTTTCCACCTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K103731-0022-062-D06C-PC35
TTTAAATTATTTCTACTATATATAAGGAAAAAAAATTGGTACCTGAAGAATGAAAGAAAC
AGAAACGAATATGCCTTGCATGGTTAGAAGAGCTCCACCGATGAGCCAATCGGAGTTTGA
AGATGACAACGGTGGAGAAAACTGACGGAAGTTTACATAGGGCGGAGAAGATGCGAGGAA
GACACGTGGACCGTGGTAGAAAATCACCACAAGTGCACCTATCAGTGACAATATCGTCCC
CATCAATTTGGCTAGACTACTCCTCTCTTTGAAAGATACTTTTTCCATTCTTTATTCACA
AAATGAAATTTAGTGTGTTAAGTGTTTATTCCAAAAGAAGGCTAAGAAATAATCAATGAT
CAAACACAATTAATACGCATTTATTTCATTCATGCAAACAGCCTTGTATGATTTGGACCA
CAAGATTGAGCAATAGTGCGATAGTAACTTAAATCTTCATATCAATTGCCATAAATATAG
ATGCCGAAAACCTGAATTAATGTATGCATTGAAAGAAAGAAAGAAGAACATATTAAACGC
ATTTACCATAAATATAGATTATTTACCTAGTTACTAATGAGTATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 54-K103732-0022-062-D06C-8409
GenBank Accession LN508453 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K103732-0022-062-D06C-8409
GCCATTTACATTGAATATACTCATTAGTAACTAGGTAAATAATCTATATTTATGGTAAAT
GCGTTTAATATGTTCTTCTTTCTTTCTTTCAATGCATACATTAATTCAGGTTTTCGGCAT
CTATATCTATGGCAATTGATATGAAGATTTAAGTTACTATCGACACTATTGCTCAATCTT
GTGGTCCAAATCATACAAGGCTGTTTGCATGAATGAAATAAATGCGTATTAATTGTGTTT
GATCATTGATTATATCGTAGCCTTCTTTTGGAACAAACACTTAACACACTAAATTTCGTT
TTGTGAATAAAGAATGGAAAAAGTATCTTTCAAAGAGAGGAGTAATCTAGCCCAATTGAT
GGGGACGATTTTGTCACTGACAGGTGCACTTGTGGTGATTTTCAACCACTGTCCACGTGT
CTTCCTCGCATCTTCTCCGCCATATGAAAACTTCCTTCAATTTTCTCCACCGTTGTCTTC
TTCAAACTCCTATGGACTCATCGGTGGAGCTCTTCTAACCATGCAGGGCATATCCCTTTC
TGTTTCTTTCATTCTTCAGGTACCAATTTTTTTTCCTTATATATAGTAGAAATAATTTTA
AATTTATGGTTAAGTCAAAAATTAGTCATTAAAAAAGGTGGAAACATACGAACA

Last Updated on 06.12.2018 16:11