Sequences from confirmation amplicon


Confirmation sequences for line 062G02 and hit At4g28556 are shown.
Line ID 062G02
Locus At4g28556 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14115257
Primers used for PCR YL02 - 8474, show primer details
   
Sequence Name 13-K024601-022-062-G02C-8409
GenBank Accession LN491867 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K024601-022-062-G02C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCTACCGTTCATTTCA
AACATATCTACATATATTAAAAATTAACGTTATGTTATAAAACCTTTGGAAGATGAACGA
TTTCAAGAGTTCTCCTGTAATGGAATCAATTCAAGGATTAGGAGAAGACGATTCCTCAGT
GAAATGTCAATCAGAGTTTGGTGGTCGGACTAGAGATTTACCAAAACTACCAAAATCCAC
GAGAAAATCATCGTCTGAGAAAGGTTCTCCGACCAAAGAAAGATCAGACAAGACCAAACG
TCGGACTTCAAACAAAGGCACATCATCGTCTAGGAGAACCAAAGACGAAGA
   
Sequence Name 14-K024601-022-062-G02C-YL02
GenBank Accession LN492337 [GenBank]
Sequencing primer gene specific primer, YL02: 5'-TGAGGTAGAATCTTCGTCTTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K024601-022-062-G02C-YL02
CCTAGACGATGATGTGCCTTTGTTTGAAGTCCGACGTTTGGTCTTGTCTGATCTTTCTTT
GGTCGGAGAACCTTTCTCAGACGATGATTTTCTCGTGGATTTTGGTAGTTTTGGTAAATC
TCTAGTCCGACCACCAAACTCTGATTGACATTTCACTGAGGAATCGTCTTCTCCTAATCC
TTGAATTGATTCCATTACAGGAGAACTCTTGAAATCGTTCATCTTCCAAAGGTTTTATAA
CATAACGTTAATTTTTAATATATGTAGATATGTTTGAAATGAACGGTAGGACAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACAT

Last Updated on 06.12.2018 16:11