Sequences from confirmation amplicon


Confirmation sequences for line 063D01 and hit At2g28850 are shown.
Line ID 063D01
Locus At2g28850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12383567
Primers used for PCR MI45 - 8474, show primer details
   
Sequence Name 81-K032614-022-063-D01C-8409
GenBank Accession LN514010 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K032614-022-063-D01C-8409
CGCACCATCTGATCGGACCCTCTTGAAATCAAACATCGAGGAGAACATGGCAATGAAGAG
CACGAGGTGGTTCAGCGCGTAACGTTGGCCCACGCATTGGTGCGAGCCAATTCCAAAAGT
TAGGAAATTGCGTTTGAACACCTCATCCTCTTGCCTTGTCTCGCTAAACCGGTCAGGATC
GAACCGGTCTGGTTCAGTAAACCCTTGAAAAGAGGCGTCAAAAAGGGAAGGAAACACAAT
TGTACCTTTTGGGATTGTGTACGATTCCGTGAGACGGAAGTCACTAACAGCAACATGTGG
GACCATACTTGCTGGTGGTCGGTATCTTAAGACCTCACGCGCCACAGCCCGAATGTACTT
CATCTCCGCGAGCTGATCGGCTGTGATCGACTCCTTGGACTCAGGCGACCAAAATCTTGC
AACGTCCTCCCTCACTCTTCTTAGCACTTCTGGCTCGGACTCAAGCAGCACCACTGCCCA
GAGGAGTGATGACGTAGACGCGTCCTGTGAGGCAAACAGAAAATCAAC
   
Sequence Name 81-K032614-022-063-D01C-MI45
GenBank Accession LN514011 [GenBank]
Sequencing primer gene specific primer, MI45: 5'-TTAGTTGTGTGCTCGTTGATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K032614-022-063-D01C-MI45
TCATCACTCCTCTGGGCAGTGGTGCTGCTTGAGTCCGAGCCAGAAGTGCTAAGAAGAGTG
AGGGAGGACGTTGCAAGATTTTGGTCGCCTGAGTCCAAGGAGTCGATCACAGCCGATCAG
CTCGCGGAGATGAAGTACATTCGGGCTGTGGCGCGTGAGGTCTTAAGATACCGACCACCA
GCAAGTATGGTCCCACATGTTGCTGTTAGTGACTTCCGTCTCACGGAATCGTACACAATC
CCAAAAGGTACAATTGTGTTTCCTTCCCTTTTTGACGCCTCTTTTCAAGGGTTTACTGAA
CCAGACCGGTTCGATCCTGACCGGTTTAGCGAGACAAGGCAAGAGGATGAGGTGTTCAAA
CGCAATTTCCTAACTTTTGGAATTGGCTCGCACCAATGCGTGGGCCAACGTTACGCGCTG
AACCACCTCGTGCTCTTCATTGCCATGTTCTCCTCGATGTTTGATTTCAAGAGGGTCCGA
TCAGATGGTTGCGATGAGATTCAAAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACT

Last Updated on 06.12.2018 16:11