Sequences from confirmation amplicon


Confirmation sequences for line 063F04 and hit At3g18060 are shown.
Line ID 063F04
Locus At3g18060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6186302
Primers used for PCR DB06 - 8474, show primer details
   
Sequence Name 47-K027488-022-063-F04C-8409
GenBank Accession LN506081 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K027488-022-063-F04C-8409
CATTTACAATTGAATAGAATACAAATGGAGTTTACCGTCTTGGCCACCTATGACAGCTTC
GGTTCCATCAGGTGTTACTGCCAAAGCAGTGACAATGAACCCAAGGTTGATGGTTGACAC
TACTTTCCCATCACGAAGAAACACAACTCCTGACTCAAAGGTCACCAGAAGGAGATCGGG
TGAGAGTGGTGCAAGGCTTAGATCCTTTGGTTGATTTCCAATGTCGATAGACTCTTCATT
TGTGCATTGATCATCTTTATAGGAGATCCTGGATATCTGAATAAGAATCACATAGTGAAT
AAACGTGAGTTAGTTGCTTCAATAACAATAATGCTTGGAAGCAATTAGATAACCTACGAA
ATAAAAAATATTAGTCACTGCTAGTCTTACAAGGTAAAGACCATTTGGCCTTCAGTGATC
GCTCTTTTAAATTCTCTAAACCACAAGATCATTAGTATATTTTTCCAAAGTGTTAATAAT
CTTAAGAGACCATTGTGTCTTTAGAATTGCCAATATTTCAGAGAGAAAAAAACCTAATTC
ACTTGT
   
Sequence Name 48-K027488-022-063-F04C-DB06
GenBank Accession LN506398 [GenBank]
Sequencing primer gene specific primer, DB06: 5'-TTGAATGTAAGGACGAAACAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K027488-022-063-F04C-DB06
TATTGGCATTCTAAAGACACAATGGGTCTCTTAAGATTATTAACACTTTGGAAAAATATA
CTAATGATCTTGTGGTTTAGAGAATTTAAAAGAGCGATCACTGAAGGCCAAATGGTCTTT
ACCTTGTAAGACTAGCAGTGACTAATATTTTTTATTTCGTAGGTTATCTAATTGCTTCCA
AGCATTATTGTTATTGAAGCAACTAACTCACGTTTATTCACTATGTGATTCTTATTCAGA
TATCCAGGATCTCCTATAAAGATGATCAATGCACAAATGAAGAGTCTATCGACATTGGAA
ATCAACCAAAGGATCTAAGCCTTGCACCACTCTCACCCGATCTCCTTCTGGTGACCTTTG
AGTCAGGAGTTGTGTTTCTTCGTGATGGGAAAGTAGTGTCAACCATCAACCTTGGGTTCA
TTGTCACTGCTTTGGCAGTAACACCTGATGGAACCGAAGCTGTCATCGGTGGCCAAGACG
GTAAACTCCATTTGTATTCTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCAAGGACTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11