Sequences from confirmation amplicon


Confirmation sequences for line 064B09 and hit At2g31830 are shown.
Line ID 064B09
Locus At2g31830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13535012
Primers used for PCR KM35 - 8474, show primer details
   
Sequence Name 73-K032613-022-064-B09C-8409
GenBank Accession LN512827 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K032613-022-064-B09C-8409
GCCATTTACAATTGAATATGGATGTCGCAGCAGTTCCATGTGGATTTGGCCGCGCCATAG
GGAACAAGGTTTGACCTTTACAGCTATATTTCCCTTGGTTATGTCTAATACAAAATCAGG
TAGTATGATGTATGATCCAATAGTCAATGATAGTGGATTTTGATGGCTAACCTCAGATTA
GGATGAATCTTCATTGTTGATCTAACTGCTCACATATTAATGTTCCTACTTTGTCAAAAA
CAGGGAGGTGTGGGTTTGAGAATCAGAGTTTATGACCGGATTATGTGCTTTGTGAACTGT
CACTTGGCTGCTCACTTGGAGGCAGTTACTCGGAGAAACGCGGATTTTAATCACATATAT
CGGTCAATGGTCTTCTCCAAAGGACAAAGTGTATATACTGCTGCAGCTGGTATATTGCCG
TACCTGTTTTTATCTTGCTCGCTTGGCTTCTCCACGTACTTATTTTGGCTTCTTTATTCT
TCTGGCTTGCCATGGGCCCTTTCTCTTGCAGCTGGTGCTTCAACATCTGCTCAAGCGCTA
AAGAATAACCCTGTAAGAAGATTTCATATTAGCATTTACATTTTTTGTTCACAATCTCGG
TCTCAAGCTCCTGCTGTTTGTCCCTTACTAATGTTATTGTTCTTTTTCCTTGTCACCTAG
AATACAAACAACAGCACTGAAGAGGAGAAGTTCTCA
   
Sequence Name 73-K032613-022-064-B09C-KM35
GenBank Accession LN512828 [GenBank]
Sequencing primer gene specific primer, KM35: 5'-AATGAGACTTCTCCTCTTCAGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K032613-022-064-B09C-KM35
AAAGAACATACATTAGTAAGGGACAAACAGCAGGAGCTTGAGACCGAGATTGTGAACAAA
AAATGTAAATGCTAATATGAAATCTTCTTACAGGGTTATTCTTTAGCGCTTGAGCAGATG
TTGAAGCACCAGCTGCAAGAGAAAGGGCCCATGGCAAGCCAGAAGAATAAAGAAGCCAAA
ATAAGTACGTGGAGAAGCCAAGCGAGCAAGATAAAAACAGGTACGGCACTATACCAGCTG
CAGCAGTATATACACTTTGTCCTTTGGAGAAGACCATTGACCGATATATGTGATTAAAAT
CCGCGTTTCTCCGAGTAACTGCCTCCAAGTGAGCAGCCAAGTGACAGTTCACAAAGCACA
TAATCCGGTCATAAACTCTGATTCTCAAACCCACACCTCCCTGTTTTTGACAAAGTAGGA
ACATTAATATGTGAGCAGTTAGATCAACAATGAAGATTCATCCTAATCTGAGGTTAGCCA
TCAAAATCCACTATCATTGACTATTGGATCATACATCATACTACCTGATTTTGTATTAGA
CATAACCAAGGGAAATATAGCTGTAAAGGTCAAACCTTGTTCCCTATGGCGCGGCCAAAT
CCACATGGAACTGCTGCGACATCCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTC
Primers used for PCR FP11 - 3144, show primer details
   
Sequence Name 88-K035342-022-064-B09C-8409
GenBank Accession LN514824 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K035342-022-064-B09C-8409
CGGAATGAAGCATTATACAATTGAATATATCCTGATAGTTTAAACCGAAGGCGGGAAACG
ACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGG
AAGGGTCTTGCGAAGGATAGTGGGATTGTAGATTTCCTCACCCTAAAGCAGAAAGTTATA
AGATATGAGGTGACAAAAGATGATTAATTTGGAATATATGATTTAACTTTACACTCTTAA
GACAGGTGAAAGATTTTTGGTGCTATGTAAGGAAATAAAACTACTTTAGTCTGGTCCACC
TGCAATCACTCGAAAACACACAAAATGTAACACGTCTGAAATGACTTTTTTGCCTACCAA
AGAGATATTAGCAGTCCAGCCAACTGCCTTGAACCCATACGTTCGAAAGTATTTCTCTCA
TCCAGTGCATTTCCAATTGCATCAAGCCACCATTGCCCCACGGCACTTCCTTCAACCCCT
ACCTACAACCGAAACACCAATATGAGCTCAATTAAAATATGTAGGACAAACAGCATTATC
CCGATATTAAGCGTATGAATTCGCATGCAACATTTTTAAACATTCTACTGATGACAGTAT
TTCTCTTTAACTAGTGATCCTAGGTAGTACTGGACAGGTTTT
   
Sequence Name 88-K035342-022-064-B09C-FP11
GenBank Accession LN514825 [GenBank]
Sequencing primer gene specific primer, FP11: 5'-GGTTGACCAGATCATTAACCAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K035342-022-064-B09C-FP11
CTGTCAGTATACCTAGGATCACTAGTTATAGAGAAATACTGTCATCAGTAGAATGTTTAA
AAATGTTGCATGCGAATTCATACGCTTAATATCGGGATAATGCTGTTTGTCCTACATATT
TTAATTGAGCTCATATTGGTGTTTCGGTTGTAGGTAGGGGTTGAAGGAAGTGCCGTGGGG
CAATGGTGGCTTGATGCAATTGGAAATGCACTGGATGAGAGAAATACTTTCGAACGTATG
GGTTCAAGGCAGTTGGCTGGACTGCTAATATCTCTTTGGTAGGCAAAAAAGTCATTTCAG
ACGTGTTACATTTTGTGTGTTTTCGAGTGATTGCAGGTGGACCAGACTAAAGTAGTTTTA
TTTCCTTACATAGCACCAAAAATCTTTCACCTGTCTTAAGAGTGTAAAGTTAAATCATAT
ATTCCAAATTAATCATCTTTTGTCACCTCATATCTTATAACTTTCTGCTTTAGGGTGAGG
AAATCTACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCA
TTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCCGCCTTCGGTTTAAACT
ATCAGGATATATTCAATTGTAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTA
Primers used for PCR FP11 - 8474, show primer details
   
Sequence Name 24-K118534-0022-064-B09C-FP11
GenBank Accession LN497195 [GenBank]
Sequencing primer gene specific primer, FP11: 5'-GGTTGACCAGATCATTAACCAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118534-0022-064-B09C-FP11
ATCACTAGTTATAGAGAATACTGTCATCAGTAGAATGTTTAAAAATGTTGCATGCGAATT
CATACGCTTAATATCGGGATAATGCTGTTTGTCCTACATATTTTAATTGAGCTCATATTG
GTGTTTCGGTTGTAGGTAGGGGTTGAAGGAAGTGCCGTGGGGCAATGGTGGCTTGATGCA
ATTGGAAATGCACTGGATGAGAGAAATACTTTCGAACGTATGGGTTCAAGGCAGTTGGCT
GGACTGCTAATATCTCTTTGGTAGGCAAAAAAGTCATTTCAGACGTGTTACATTTTGTGT
GTTTTCGAGTGATTGCAGGTGGACCAGACTAAAGTAGTTTTATTTCCTTACATAGCACCA
AAAATCTTTCACCTGTCTTAAGAGTGTAAAGTTAAATCATATATTCCAAATTAATCATCT
TTTGTCACCTCATATCTTATAACTTTCTGCTTTAGGGTGAGGAAATCTACAATCCCACTA
TCCTTCGCAAGACCCTTCCTCTATATGAGGAAGTTCATTTCATTTGGAGAGGACAGGGTA
CCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 03-K118533-0022-064-B09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118533-0022-064-B09C-8409
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11