Sequences from confirmation amplicon


Confirmation sequences for line 064C11 and hit At2g29940 are shown.
Line ID 064C11
Locus At2g29940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12762823
Primers used for PCR BD14 - 8474, show primer details
   
Sequence Name 09-K028144-022-064-C11C-8409
GenBank Accession LN490036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K028144-022-064-C11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCTACAAATCCAACCTTTAT
GGAACACAAAATGTTTTAGATCAGAATCTTAGAATTTTATCTAAGGACATTTGAATAATA
TCTACGTCAAGATGTCACTATAGTGATGCAAATTGTATCACATAATGGTGCATATATATA
AGAATAAGACTAATTTTAGAAAAGCAGAATTGAAGCAAGCGCAAATGAAGACCTAGGCTT
ATGGTATAGACAAAATTTATTGGTTACTTGATTCAAAAGCCACACAAAACCAATGTCATG
GAAACAAACCTAAGTGTCAATACTCTAGATGATGCCAAATTTACGAATGCAGCAAAAAGA
TGTAGATATTTACCTGGCATGTCCTAAAAGTGTAAAGAAATTTGTGCCGTTTGATCAATA
AT
   
Sequence Name 10-K028144-022-064-C11C-BD14
GenBank Accession LN490511 [GenBank]
Sequencing primer gene specific primer, BD14: 5'-TTATTATTGATCAAACGGCACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K028144-022-064-C11C-BD14
CTTTAACTTTTAGGACATGCCAGGTAAATATCTACATCTTTTTGCTGCATTTGTAAATTT
GGCATCATCTAGAGTATTGACACTTAGGTTTGTTTCCATGACATTGGTTTTGTGTGGCTT
TTGAATCAAGTAACCAATAAATTTTGTCTATACCATAAGCCTAGGTCTTCATTTGCGCTT
GCTTCAATTCTGCTTTTCTAAAATTAGTCTTATTCTTATATATATGCACCATTATGTGAT
ACAATTTGCATCACTATAGTGACATCTTGACGTAGATATTATTCAAATGTCCTTAGATAA
AATTCTAAGATTCTGATCTAAAACATTTTGTGTTCCATAAAGGTTGGATTTGTAGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCACCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTC

Last Updated on 06.12.2018 16:11