Sequences from confirmation amplicon


Confirmation sequences for line 064G04 and hit At3g44340 are shown.
Line ID 064G04
Locus At3g44340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16014439
Primers used for PCR NA11 - 8474, show primer details
   
Sequence Name 04-K101668-0022-064-G04C-8409
GenBank Accession LN487857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K101668-0022-064-G04C-8409
TTCGGACTGAGCCTTTACATTGAATTATCCTGATGCAAGAATACTCAGGGAACTTCTGTA
AGCGCATACCAACTGACATCGATCTACCTGCGGTGGGTGCATCCTCCTTCAAAATTTACG
ATTAATTGTCTCTGATGCGTTCCATGTATGTGTTTATTGTGTCAATACCAAAAATGAATA
TTTCTCGTTTTTGGTTTCGGGGCTAACATTATGTTCATGTTGCTTTGTTACCTAAAACGT
ATAATATTAGTCAAGAGGGAAACACATGTTTTTGCTGCAAGTTTACCTTGGAGAAGCACT
TTGGTATAAATTTACTACATTTTTGACGAGCCTTGAGTGCCATGCAGATTGACTGTGACA
AAGCTATAATGGTGACATTAAAGCATGATGACAAACTACAAGATGGAG
   
Sequence Name 04-K101674-0022-064-G04C-NA11
GenBank Accession LN487858 [GenBank]
Sequencing primer gene specific primer, NA11: 5'-GCTCCATCTTGTAGTTTGTCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K101674-0022-064-G04C-NA11
GCTCGTCAAAAATGTAGTAAATTTATACCAAAGTGCTTCTCCAAGGTAAACTTGCAGCAA
AAACATGTGTTTCCCTCTTGACTAATATTATACGTTTTAGGTAACAAAGCAACATGAACA
TAATGTTAGCCCCGAAACCAAAAACGAGAAATATTCATTTTTGGTATTGACACAATAAAC
ACATACATGGAACGCATCAGAGACAATTAATCGTAAATTTTGAAGGAGGATGCACCCACC
GCAGGTAGATCGATGTCAGTTGGTATGCGCTTACAGAAGTTCCCTGAGTATTCTTGCATC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11