Sequences from confirmation amplicon


Confirmation sequences for line 065E11 and hit At3g27610 are shown.
Line ID 065E11
Locus At3g27610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10226674
Primers used for PCR 03uj - 8474, show primer details
   
Sequence Name 47-K117452-0022-065-E11C-03uj
GenBank Accession LN506318 [GenBank]
Sequencing primer gene specific primer, 03uj: 5'-TGACTGATTCTGAGGTGCTTAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117452-0022-065-E11C-03uj
AAAAAAAACAGAACAGCTTATAGATGGACAAATATGCTCTAAGATCTATCCATTTTCTAA
AGGTAAGATTTTGGTTATTTGACTCAATAAAACTTACTTTGAAGGTTTTTTCAACTGTTT
CTTGCATTGGCCATTAATGATGTAGTTTTATACCTTTATAGCAGAATCATACGGGTCGGA
TAAAGATAGAAAGATTATACTGCCTGGTTCTTTTAACCCATTACATGACGGTGATCTCAA
GCTGTTGGAAGCTGCTATGAGGTAAGCTAGTCTGTCTTCATTATTTAATCATTATTTTCT
TTGACTCATTGTTGATTTTGGTAAGTTTTAATCATGTAGATTTCATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 24-K117451-0022-065-E11C-8409
GenBank Accession LN497163 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117451-0022-065-E11C-8409
TGAATGAAATCTACATGATTAAAACTTACCAAAATCAACAATGAGTCAAAGAAAATAATG
ATTAAATAATGAAGACAGACTAGCTTACCTCATAGCAGCTTCCAACAGCTTGAGATCACC
GTCATGTAATGGGTTAAAAGAACCAGGCAGTATAATCTTTCTATCTTTATCCGACCCGTA
TGATTCTGCTATAAAGGTATAAAACTACATCATTAATGGCCAATGCAAGAAACAGTTGAA
AAAACCTTCAAAGTAAGTTTTATTGAGTCAAATAACCAAAATCTTACCTTTAGAAAATGG
ATAGATCTTAGAGCATATTTGTCCATCTATAAGCTGTTCTAGTTCTTCTTCTTCACTAAA
CTGGATTACAGACTCATTAAGCACCTCAAAATCAGTC

Last Updated on 06.12.2018 16:11