Sequences from confirmation amplicon


Confirmation sequences for line 065F04 and hit At3g21250 are shown.
Line ID 065F04
Locus At3g21250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7459959
Primers used for PCR FV35 - 8474, show primer details
   
Sequence Name 75-K033880-022-065-F04C-FV35
GenBank Accession LN513181 [GenBank]
Sequencing primer gene specific primer, FV35: 5'-AGTAGCAGGAAATGCAAAAACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K033880-022-065-F04C-FV35
GTTTGATATTACTGACTGATGATCTTGATCAGGTTATGGAGGAAGGAACAATCACTCAGT
CAGGAAAATATGAGGAGCTCTTAATGATGGGGACTGCATTCCAACAGCTGGTAAATGCTC
ATAATGATGCAGTAACTGTATTACCTTTAGCGAGTAACGAAAGCCTCGGAGATCTTAGGA
AAGAGGGCAAGGACAGAGAGATAAGAAACATGACGGTTGTCGAAAAAATCGAAGAAGAGA
TCGAAAAAACGGATATTCCAGGGGTACAACTTACACAAGAAGAAGAAAAAGAGAGCGGTT
ATGTTGGGATGAAACCTTTCTTGGACTATATCGGTGTCTCTCGAGGATGGTGTCTTCTAT
GGTCAAGCGTATTGGGTCAGGTTGGCTTTGTAGTTTTTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAAATG
   
Sequence Name 19-K033880-022-065-F04C-8409
GenBank Accession LN494665 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K033880-022-065-F04C-8409
TGAAGCCATTTACAATTGAAAAACTACAAAGCCAACCTGACCCAATACGCTTGACCATAG
AAGACACCATCCTCGAGAGACACCGATATAGTCCAAGAAAGGTTTCATCCCAACATAACC
GCTCTCTTTTTCTTCTTCTTGTGTAAGTTGTACCCCTGGAATATCCGTTTTTTCGATCTC
TTCTTCGATTTTTTCGACAACCGTCATGTTTCTTATCTCTCTGTCCTTGCCCTCTTTCCT
AAGATCTCCGAGGCTTTCGTTACTCGCTAAAGGTAATACAGTTACTGCATCATTATGAGC
ATTTACCAGCTGTTGGAATGCAGTCCCCATCATTAAGAGCTCCTCATATTTTCCTGACTG
AGTGATTGTTCCTTCCTCCATAACCTGATCAAGATCATCAGTCAGTAATAATCAAACGGG
TAAGACAAAGACAATATTGTTTTTGCATTTCCTGCTA
Primers used for PCR FV36 - 3144, show primer details
   
Sequence Name 76-K033880-022-065-F04C-FV36
GenBank Accession LN513335 [GenBank]
Sequencing primer gene specific primer, FV36: 5'-TTCAAAGAAGAAGCGGATGTTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K033880-022-065-F04C-FV36
TTTGCTGCATAAACAACTAAAAACAGAAGTAGAATCGAATCATGTACCTGAACAACTTTN
CGTGGCTGCCAAAGCAAGAAGGGCTATGATAATTACTTGCCATGTGACATACGTCATGAT
GAGAAGAGCTGCAGTGAGCTCAACAGCCGGTGCTACCACAAATATGAAGGCAAATGGAAC
GTCATAGTCCAACACGTTCAAATCAGACGAAGCCTGCACATGTTACATTCAACTATATCA
TACATTGAGCTGTAGTGTATTCCATATAATTATAAGCAAAGAGATCTTACTCGGGTGAGA
ATGCGGCCAACTGGAGTAGAATCAAAGAAAAGCATCGGGGCTTTGAAGACTGCATTTGTG
AAACCAGAGAAGAAGGCTTTGGAAGCTTTTAGTCCAAGATGGGCAGTCGTAATAGCTCTC
GCATATACAAAACCAGCACTAAGAGTTGATATAATGCTATAGACTCCAATAAGCATTGTA
TTCGTGATTTTAGGGATCCCGATGGCAAAAGCCAGCCAGTATGTTGAAATCTACATGTAA
AGGCGGAAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAA
ATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC
ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAAC
GTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGG
   
Sequence Name 20-K033880-022-065-F04C-3144
GenBank Accession LN495120 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K033880-022-065-F04C-3144
TCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGG
ACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTTCCGCCTTTACATGTA
GATTTCAACATACTGGCTGGCTTTTGCCATCGGGATCCCTAAAATCACGAATACAATGCT
TATTGGAGTCTATAGCATTATATCAACTCTTAGTGCTGGTTTTGTATATGCGAGAGCTAT
TACGACTGCCCATCTTGGACTAAAAGCTTCCAAAGCCTTCTTCTCTGGTTTCACAAATGC
AGTCTTCAAAGCCCCGATGCTTTTCTTTGATTCTACTCCAGTTGGCCGCATTCTCACCCG
AGTAAGATCTCTTTGCTTATAATTATATGGAATACACTACAGCTCAATGTATGATATAGT
TGAATGTAACATGTGCAGGCTTCGTCTGATTTGAACGTGTTGGACTATGACGTTCCATTT
GCCTTCATATTTGTGGTAGCACCGGCTGTTGAGCTCACTGCAGCTCTTCTCATCATGACG
TATGTCACATGGCAAGTAATTATCATAGCCCTTCTTGCTTTGGCAGCCACGAAAGTTGTT
CAGGTACATGATTCGATTCTACTTCTGTTTTTAGTTGTTTTATGCAGCAAATAAGTCTGT
ATTAACATCCGCTTCTTTCTTTGAAAAAAAAAAAAAAGT

Last Updated on 06.12.2018 16:11