Sequences from confirmation amplicon


Confirmation sequences for line 066B08 and hit At1g55120 are shown.
Line ID 066B08
Locus At1g55120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20568508
Primers used for PCR 01ew - 3144, show primer details
   
Sequence Name 21-K110678-0022-066-B08C-01ew
GenBank Accession LN495709 [GenBank]
Sequencing primer gene specific primer, 01ew: 5'-GAATCAGGAAAGGAATTGCTACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K110678-0022-066-B08C-01ew
GCACAAGTCAACTGGCAAAGAAAGTTCTCAAAGCAGGATCTACTCTCCAAGTTCATGGTG
TCACTGCTGCGCAGGTATGACTCAAACCTTATTCCTTAAACTTGGCCATATCAATGGTGA
ATCCGAATCTAGTATTGGGAATTGACCTAAAACTACTTAGTTTTTCTTTTAGCAAAGTGT
GTTTCTTGCTTATTTTGTTTTTGGTATTGAGCAGGCAGATGTTGAGGTATCGTTCAAGGT
GAAGGAATTGGAAAAGGCGGATGTGATTGAACCGAGTTGGACCGATCCACAAAAGATATG
TAGTCAGGGAGATTTATCGGTTATGTCGGGTTTAGGACCATTTGGTTTGATGGTTTTGGC
ATCCAATGACATGGAAGAGTACACTCAAAAATGGCTTAAGAGGAAGGGTCTTGCGAAGGA
TAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATC
   
Sequence Name 05-K110677-0022-066-B08C-3144
GenBank Accession LN488407 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K110677-0022-066-B08C-3144
ATCCACTATCTTGCAGACCCTTCCTCTAAGCCATTTTTGAGTGTACTCTTCCATGTCATT
GGATGCCAAAACCATCAAACCAAATGGTCCTAAACCCGACATAACCGATAAATCTCCCTG
ACTACATATCTTTTGTGGATCGGTCCAACTCGGTTCAATCACATCCGCCTTTTCCAATTC
CTTCACCTTGAACGATACCTCAACATCTGCCTGCTCAATACCAAAAACAAAATAAGCAAG
AAACACACTTTGCTAAAAGAAAAACTAAGTAGTTTTAGGTCAATTCCCAATACTAGATTC
GGATTCACCATTGATATGGCCAAGTTTAAGGAATAAGGTTTGAGTCATACCTGCGCAGCA
GTGACACCATGAACTTGGAGAGTAGATCCTGCTTTGAGAACTTTCTTTTGCCAGTTGACT
TGTTGCCCACGCAATGTCTCAATCTCTTCAATCGGCCACTGTAGCAATTC

Last Updated on 06.12.2018 16:11