Sequences from confirmation amplicon


Confirmation sequences for line 066E01 and hit At2g34490 are shown.
Line ID 066E01
Locus At2g34490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14536116
Primers used for PCR MS44 - 8474, show primer details
   
Sequence Name 53-K017405-022-066-E01C-MS44
GenBank Accession LN507988 [GenBank]
Sequencing primer gene specific primer, MS44: 5'-ACGTTTCTAGAATCTCATCCGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K017405-022-066-E01C-ms44
GAAGTGGCCAAGATTTGGTCACCTCAGTCTGGCCATCTGATCACGGCCGATCAGCTCGCG
GAGATGAAGTATACTCGCGCTGTGGCACGTGAGGTGGTGAGATATCGACCACCGGCAACT
ATGGTCCCACACATTGCTACTAATGATTTCCCTCTTACAGAATCGTACACTATCCCAAAA
GGTACAATAGTGTTTCCCTCGGTTTTCGACGCCTCGTTTCAAGGGTTTACTGAACCGAAC
CGGTTCGATCCGGACCGGTTTAGTGAGACAAGGCCAGCGTATACCTACGGACAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACATTCAC
   
Sequence Name 57-K017405-022-066-E01C-8409
GenBank Accession LN509264 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K017405-022-066-E01C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCGTAGGTATACGCT
GGCCTTGTCTCACTAAACCGGTCCGGATCGAACCGGTTCGGTTCAGTAAACCCTTGAAAC
GAGGCGTCGAAAACCGAGGGAAACACTATTGTACCTTTTGGGATAGTGTACGATTCTGTA
AGAGGGAAATCATTAGTAGCAATGTGTGGGACCATAGTTGCCGGTGGTCGATATCTCACC
ACCTCACGTGCCACAGCGCGAGTATACTTCATCTCCGCGAGCTGATCGGCCGTGATCAGA
TGGCCAGACTGAGGTGACCAAATCTTGGCCACTTCCTCCCTCACTTTGCTCAGCACTTTC
GGATGAGAATTC

Last Updated on 06.12.2018 16:11