Sequences from confirmation amplicon


Confirmation sequences for line 066H03 and hit At1g70600 are shown.
Line ID 066H03
Locus At1g70600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26621613
Primers used for PCR KA12 - 8474, show primer details
   
Sequence Name 24-K105138-0022-066-H03C-KA12
GenBank Accession LN496987 [GenBank]
Sequencing primer gene specific primer, KA12: 5'-GCTTCCTTAATCTTCTTCTCAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K105138-0022-066-H03C-KA12
ACGGCTTGTTCTCGGCAATGACCTTTACCCAAAACCTTGAAGAAACTATGTTGAGTCACA
TCGATCAACGGAACATTGTCCTTGGTTGATTTCGCCTTCACATCTTCAGGGACGAGTGAC
CAAAGCTTGTCGAGGTTAACGATTGGGCAGAAGAACTTGTTCCTCAGCTTGTGGAAATAC
CTCATACCAACTTTACCGAAGTAACCTGGATGGTACTTGTCGAAGAGGATCCTGTGGTGA
TGCATACCTCCAGCGTTACCACGACCTCCGGGATGCTTACGGTGCTTACCGATACGTCCA
TGTCCGGCGCTGACGTGACCTCTCTTCTTCCTGTTCTTCTTGAATCTGGTTGTCATTTTC
GTCTATATCTGCTGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACGATTTATT
   
Sequence Name 08-K105139-0022-066-H03C-8409
GenBank Accession LN489725 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K105139-0022-066-H03C-8409
CATTTACATTGAATATATCCTGCCAGCAGATATAGACGAAAATGACAACCAGATTCAAGA
AGAACAGGAAGAAGAGAGGTCACGTCAGCGCCGGACATGGACGTATCGGTAAGCACCGTA
AGCATCCCGGAGGTCGTGGTAACGCTGGAGGTATGCATCACCACAGGATCCTCTTCGACA
AGTACCATCCAGGTTACTTCGGTAAAGTTGGTATGAGGTATTTCCACAAGCTGAGGAACA
AGTTCTTCTGCCCAATCGTTAACCTCGACAAGCTTTGGTCACTCGTCCCTGAAGATGTGA
AGGCGAAATCAACCAAGGACAATGTTCCGTTGATCGATGTGACTCAACATGGTTTCTTCA
AGGTTTTGGGTAAAGGTCATTTGCCTGAGAACAAGCCGTTCGTTGTGAAGGCGAAGCTTA
TTTCGAAGACTGCTGAGAAG

Last Updated on 06.12.2018 16:11