Sequences from confirmation amplicon


Confirmation sequences for line 067A09 and hit At5g59800 are shown.
Line ID 067A09
Locus At5g59800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24095786
Primers used for PCR GE34 - 8474, show primer details
   
Sequence Name 40-K033737-022-067-A09C-8409
GenBank Accession LN503622 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K033737-022-067-A09C-8409
TTACATTGAATATATCCTGATCCAGAAGAACCATATGCTTCTGTAATTTCAATCAGAACA
AAGGACCTGTTTGGTTCTTGCTCCTAAATAAACCCTTGTCTTGTCCTGGAAAAAGGATCC
TGCTCGAGTCAAGCGTCTGTCATTTACTTCTCCAGCCAAGTGTCTGTGAATAGCTTCTAG
TGATGAAAACTGTCTACCAGTCCCTGGCTCGATAAAGTACTGCCCACAAAACAAAACAAA
AACAAGTAAAGATGAGACTTTTCAGAAGAGAACCACAAGATCTATCTTCAACTCAAGCAT
ATACCCAAAGAAACGACTCATAGACCTCTCAAGCAACAATCAAGAGCTCTGTGGCAACAC
AATCACTAATCTGATCCAAGCATTACACTTTGGTCATACATATCCATTGCACAAAAGACA
TAAATTAGCTCTAATTGTTCAAATTCTGAGAAAAAGCTCAAATTTTGTTTCACATTTGCT
TGATGTAGCTCATATTTACCGTATCAACAACACCGTTGTTGCTAGATCGCGGCCGATTCA
CGACGGTCCAACCAGGCATAATCTTGCCGCAAAACGAGGTGGGATCCGCGATCTGCAACT
GGGTTTCGCGGCGGTGGTTAGCCGAAGGAGAGGAAGAGGATCTCGTCTGCATCGCTTCGT
TGCCGGGTCTAACCCAAAAGCCCGAGACGAGAGTGTGGTTTTTAGTTTGGTCGTCCAAGC
CACACACA
   
Sequence Name 42-K033737-022-067-A09C-GE34
GenBank Accession LN504369 [GenBank]
Sequencing primer gene specific primer, GE34: 5'-GAAATAAGAAGTGTGTGTGGCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K033737-022-067-A09C-GE34
ACACACTCTCGTCTCGGGCTTTTGGGTAGACCCGGCAACGAAGCGATGCAGACGAGATCC
TCTTCCTCTCCTTCGGCTAACCACCGCCGCGAAACCCAGTTGCAGATCGCGGATCCCACC
TCGTTTTGCGGCAAGATTATGCCTGGTTGGACCGTCGTGAATCGGCCGCGATCTAGCAAC
AACGGTGTTGTTGATACGGTAAATATGAGCTACATCAAGCAAATGTGAAACAAAATTTGA
GCTTTTTCTCAGAATTTGAACAATTAGAGCTAATTTATGTCTTTTGTGCAATGGATATGT
ATGACCAAAGTGTAATGCTTGGATCAGATTAGTGATTGTGTTGCCACAGAGCTCTTGATT
GTTGCTTGAGAGGTCTATGAGTCGTTTCTTTGGGTATATGCTTGAGTTGAAGATAGATCT
TGTGGTTCTCTTCTGAAAAGTCTCATCTTTACTTGTTTTTGTTTTGTTTTGTGGGCAGTA
CTTTATCGAGCCAGGGACTGGTAGACAGTTTTCATCACTAGAAGCTATTCACAGACACTT
GGCTGGAGAAGTAAATGACAGACGCTTGACTCGAGCAGGATCCTTTTTCCAGGACAAGAC
AAGGGTTTATGAAGGATCAAGAACCAAACAGGTCCTTTGTTCTGATTTGAAATTACAGAA
GCATATGGTTCTTCTTGGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTG

Last Updated on 06.12.2018 16:11