Sequences from confirmation amplicon


Confirmation sequences for line 067F03 and hit At5g53890 are shown.
Line ID 067F03
Locus At5g53890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21879894
Primers used for PCR KE26 - 8474, show primer details
   
Sequence Name 49-K103548-0022-067-F03C-KE26
GenBank Accession LN506811 [GenBank]
Sequencing primer gene specific primer, KE26: 5'-CCATAAGTCTAGCCATTGGGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K103548-0022-067-F03C-KE26
TTAGATTTCAGANAGATGTGGATGATCGAATCAATGACGTTGATGAGGAGACTATCAGCG
GGGTTTCGAAAGCTCTCGGGCCATCAAAGATTGTGCTTTTCCATAGCTGTGGATGTAAAG
ATCTAAGTGTTGAGGAGTTGTTGAAGTCTACGAACAATTTCAGCCAGGCTAACATTATAG
GATGTGGCGGATTTGGTCTTGTGTACAAAGCTAATTTTCCCGATGGCTCGAAAGCAGCAG
TCAAGAGGCTTTCTGGTGACTGTGGGCAGATGGAACGTGAATTCCAAGCGGAAGTTGAAG
CATTGTCTCGAGCGGAACATAAGAATCTAGTCTCTCTTCAAGGCTACTGCAAGCATGGAA
ACGATAGGCTGCTTATTTACTCGTTTATGGAGAATGGAAGTTTGGATTATTGGCTGCATG
AGCGGGTAGATGGGAATATGACTCTTATATGGGATGTGAGATTGAAGATAGCTCAAGGCG
CAGCGCGAGGGCTTGCTTACTTGCATAAAGTCTGTGAACCTAATGTTATACATAGGGATG
TGAAGTCTACATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCC
   
Sequence Name 33-K103549-0022-067-F03C-8409
GenBank Accession LN500963 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K103549-0022-067-F03C-8409
TTACAATTGAATATATCCATGTAGACTTCACATCCCTATGTATAACATTAGGTTCACAGA
CTTTATGCAAGTAAGCAAGCCCTCGCGCTGCGCCTTGAGCTATCTTCAATCTCACATCCC
ATATAAGAGTCATATTCCCATCTACCCGCTCATGCAGCCAATAATCCAAACTTCCATTCT
CCATAAACGAGTAAATAAGCAGCCTATCGTTTCCATGCTTGCAGTAGCCTTGAAGAGAGA
CTAGATTCTTATGTTCCGCTCGAGACAATGCTTCAACTTCCGCTTGGAATTCACGTTCCA
TCTGCCCACAGTCACCAGAAAGCCTCTTGACTGCTGCTTTCGAGCCATCGGGAAAATTAG
CTTTGTACACAAGACCAAATCCGCCACATCCTATAATGTTAGCCTGGCTGAAATTGTTCG
TAGACTTCAACAACTCCTCAACACTTAGATCTTTACATCCACAGCTATGGAAAAGCACAA
TCTTTGATGGCCCGAGAGCTTTCGAAACCCCGCTGATAGTCTCCTCATCAACGTCATTGA
TTCGATCATCCACATCTTTTCTTGAAATCCTTAACAGAATAACAGAAAGAAGTAGAGTAA
TCCCAATG
Primers used for PCR TN16 - 3144, show primer details
   
Sequence Name 44-K106973-0022-067-F03C-3144
GenBank Accession LN505197 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K106973-0022-067-F03C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAT
GAGAAGTTTGAAGCTCATCTTGCGGATTTTGGGTTAGCGAGGTTGCTTAGGCCGTATGAT
ACTCACGTGACGACTGATTTGGTTGGGACATTGGGTTATATTCCTCCTGAGTATAGCCAG
TCTTTGATTGCAACATGTAGAGGAGACGTTTACAGTTTTGGCGTTGTGCTTTTGGAGCTA
GTTACGGGTCGTAGACCTGTAGAAGTCTGTAAAGGGAAAAGTTGCAGAGATTTGGTGTCT
CGGGTGTTTCAAATGAAGGCTGAGAAGCGTGAAGCTGAGCTTATCGATACAACAATACGC
GAAAATGTGAACGAGAGAACGGTTTTGGAGATGTTGGAGATTGCTTGCAAATGCATTGAT
CATGAGCCTAGAAGGAGACCACTGATCGAAGAAGTCGTTACTTGGCTTGAAGATCTTCCT
ATGGAGTCTGTCCNAACAAC
   
Sequence Name 60-K106974-0022-067-F03C-TN16
GenBank Accession LN510272 [GenBank]
Sequencing primer gene specific primer, TN16: 5'-TTGTTGTTGAACAGACTCCATAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K106974-0022-067-F03C-TN16
GACTTCTTCGATCAGTGGTCTCCTTCTAGGCTCATGATCAATGCATTTGCAAGCAATCTC
CAACATCTCCAAAACCGTTCTCTCGTTCACATTTTCGCGTATTGTTGTATCGATAAGCTC
AGCTTCACGCTTCTCAGCCTTCATTTGAAACACCCGAGACACCAAATCTCTGCAACTTTT
CCCTTTACAGACTTCTACAGGTCTACGACCCGTAACTAGCTCCAAAAGCACAACGCCAAA
ACTGTAAACGTCTCCTCTACATGTTGCAATCAAAGACTGGCTATACTCAGGAGGAATATA
ACCCAATGTCCCAACCAAATCAGTCGTCACGTGAGTATCATACGGCCTAAGCAACCTCGC
TAACCCAAAATCCGCAAGATGAGCTTCAAACTTCTCATCAAACACTGATAGTTTAAACCG
AAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTT
CCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCA
GTGGAGATAT

Last Updated on 06.12.2018 16:11