Sequences from confirmation amplicon


Confirmation sequences for line 068A01 and hit At1g74770 are shown.
Line ID 068A01
Locus At1g74770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28089835
Primers used for PCR 03tg - 3144, show primer details
   
Sequence Name 37-K116770-0022-068-A01C-03tg
GenBank Accession LN502754 [GenBank]
Sequencing primer gene specific primer, 03tg: 5'-GGGTATTGACTACTTCCACTGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K116770-0022-068-A01C-03tg
TTAGAAGATAATTGTCCGATTTGCCATGAGTATATCTTCACCTCCAGCTCCCCAGTGAAG
GCTCTTCCTTGTGGTCACTTGATGCACTCCACATGCTTTCAGGTACTTAACCCTGTCAAA
ATATATTTTACGTTAGTAATGAAAAAAGTAAAACACATGGTAATGCTAAGATTACTTAGG
CTTTTTTCTTTGATTCTGGTTGTAGGAGTACACATGTTCACATTACACATGCCCTGTCTG
CAGCAAGTCGCTTGGAGATATGCAGGTCAGTTACACCATTTTAAAGCAGAGTATTGTTTT
CCCTATAAAACATTCACTAAACAAAAACCTTTCTCACACAGGTCTACTTTAAGATGTTAG
ACGCATTGCTTGCAGAAGAGAAGATGCCTGATGAATACTCCAACAAAACTCAGGTTTAGA
TCTTTCTCTATCTCTCTTCATAATGTTTACATAGTACAAAGCATCTCT
   
Sequence Name 13-K116769-0022-068-A01C-3144
GenBank Accession LN492217 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K116769-0022-068-A01C-3144
CTTCCAGACCATTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCACCCTCAAAGATGATACGACCCTAATTTATTACATTTGGAAT
ATTTTTTATATTTTATTTTTGTACATCATCAAATGTTACAAAAATGTTTTCTTACCAGCC
ATGTACTACATAGACGGCTGGTTCTACAATGATGTCAATGGAAAAGGAAAATTGGAGAAA
AATCTAAAGGAGCCTTGAGTTGTAGGAGCCACAGGTTGTGCATTTGTGGTAAAGCCAATG
GTAAGGAGCATTTCCTTTTCTTCCACAATCATTACACAAGATCACCTGCACCAAACCATT
TTTCCATTCAGCTTATTCTTCAGAGAGATGCTTTGTACTATGTAAACATTATGAAGAGAG
ATAGAGAAAGATCTAAACCTGAGTTTTGTTGGAGTATTCATCAGGCATCTTCTCTTCTGC
AAGCAATGCGTCTAACATCTTAAAGTAGACCTGTGTGAGAAAGGTTTTTGTTTAGTGAAT
GTTTTATAGGGAAAACAATACTCTGCTTTAAAATGGTGTAACTGACCTGCATATCTCCAA
GCGACTTGCTGCAGACAGGGCATGTGTAATGTGAACATGTGTACTCCTACAACCAGAATC
AAAGAAAAAAGCCTAAGTAATCTTAGCATTACCATGTGTTTTACTTTTTTCATTACTAAC
GTAAAATATATTTTGACAGGGTTAAGTACCTGAAAGCATGTGGAGTGCATCAAGTGACCA
CAAGGAAGAGCCTTCACTGGGGAGCTGGAGGTGAAGATATACTCATGGCAAATCGGACAA
TTATCTTCTAAGCACTTTTCCCTGCAAACATGCTCTACTAAAGTACGTGACATACAAAAA
TTGCATTTCATGCAGTG

Last Updated on 06.12.2018 16:11