Sequences from confirmation amplicon


Confirmation sequences for line 068D09 and hit At1g07520 are shown.
Line ID 068D09
Locus At1g07520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2311977
Primers used for PCR FB06 - 8474, show primer details
   
Sequence Name 86-K034733-022-068-D09C-FB06
GenBank Accession LN514612 [GenBank]
Sequencing primer gene specific primer, FB06: 5'-CGACATATTCAATTTTGGCAACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K034733-022-068-D09C-FB06
ATTATAAGCTAGTATCACAACATATGGATCATTTAAGCTTCGATAAGCGGTCAACATATG
GGGGAATTAGGAGACAAATCACTACTTAAAAAGAACCACTAGTCATTACTTTTTGTGGAT
AACATGACAATCACTACAATAAAAACTGGGCCCATCAAGCCCAAGAATACTCTTAATGAC
AAGTCAAAAGAGAGATAGTTTTGAGTTTTGACTCCTCGGACGAATCGCAAGACTGGTCTT
GTCCCCCTTTCGAGTTTCCCGGGAAACTTCCTCCTTCCTCTAATCTCTCTACAGTTCTCA
ATTTCTTGCTTCTTCTCTCACGCTTAGCAATTATACCCAATCCCATTTGAATATTACTCT
TCGCCAACATGTCCTTAAATTATCTCTTTTTAATGAATTGATCGATAAAGTTCTCACCTT
TATGCCTCCTCCTACTTTTTTCCACTCAACAATTATGAATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 85-K034733-022-068-D09C-8409
GenBank Accession LN514498 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K034733-022-068-D09C-8409
GGACTGAGCCATTTACATTGAATATTCATAATTGTTGAGTGGAAAAAAGTAGGAGGAGGC
ATAAAGGTGAGAACTTTATCGATCAATTCATTAAAAAGAGATAATTTAAGGACATGTTGG
CGAAGAGTAATATTCAAATGGGATTGGGTATAATTGCTAAGCGTGAGAGAAGAAGCAAGA
AATTGAGAACTGTAGAGAGATTAGAGGAAGGAGGAAGTTTCCCGGGAAACTCGAAAGGGG
GACAAGACCAGTCTTGCGATTCGTCCGAGGAGTCAAAACTCAAAACTATCTCTCTTTTGA
CTTGTCATTAAGAGTATTCTTGGGCTTGATGGGCCCAGTTTTTATTGTAGTGATTGTCAT
GTTATCCACAAAAAGTAATGACTAGTGGTTCTTTTTAAGTAGTGATTTGTCTCCTAATTC
CCCCATATGTTGACCGCTTATCGAAGCTTAAATGATCCATATGTTTGTTGATACTAGCTT
ATAATTTTGTTTGCTTCAAGAAAGTAGTTGCCAAAATTGA

Last Updated on 06.12.2018 16:11