Sequences from confirmation amplicon


Confirmation sequences for line 068E01 and hit At4g24390 are shown.
Line ID 068E01
Locus At4g24390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12614893
Primers used for PCR ME03 - 8474, show primer details
   
Sequence Name 19-K023014-022-068-E01C-8409
GenBank Accession LN494583 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K023014-022-068-E01C-8409
GATTTCCCGGACATGAAGCCATTTACTCATTTACATTTACATATATCCCGGATATTGGAG
GTATTCCGGTCTAAATTCCCTGAATCCTGAGAGACAAACTATGGATTTACAAGCACGAAA
AGCAGCTGCATAATCCGGTTGTTGTTCTCCCTGAGGCACATTGTCTGGACTAAATGACCC
CGTCCCAAGACTCGTTAACTGCGGCGCTCGAACCATTAGTCGATGCAGCTCTTCAAGGGA
AACAAACCTGTTCGTTCTAAGTTTCTTCAAGAATGGTGACCTAACCACGAGCTCCTCCAA
TGCCTTGAAATTGATAGGGGATTCAACACAGTCAAAAGACAAAGACTCCAGATGAGTTTC
ACCCTCAGGAAAACAAGAAATCCAATCCAACTCATCATCTGTGACTTCTGACTCCATCAA
ATCAAGGACCTTTAGCTGCCTGAAAACAAAGTCCCACTTGATAAGCATTTCTAGAAGACT
CTGAGAACAAACAAACAAACAAACAAAGAGAAGAGATCTACAAAGTACCTGCATTTGTTA
GCAACAATAGCAATACCACTAGTCCCAAAACCTTCACAGCAGACCAAAGTAAGCTCTTTG
AACCCAGGAAACGACTCAGCAAGAAGAGCCAAATCATCATCCGTAACAAACATACGCTTC
AAATGAACCTTCTCGAGCCAAGGATAAGCCTTAGCTGTAGCAGCAACCCAAGGAGAGAAT
TGAGCTCCCCAATTAGGAGGCATGAGATTAAAATCAGCAAACCTAGGTTTCCCTTT
   
Sequence Name 20-K023014-022-068-E01C-ME03
GenBank Accession LN495039 [GenBank]
Sequencing primer gene specific primer, ME03: 5'-TTAGGTCTCTTGTGCTTAAAGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K023014-022-068-E01C-ME3
GTTTGCTGATTTTAATCTCATGCCTCCTAATTGGGGAGCTCAATTCTCTCCTTGGGTTGC
TGCTACAGCTAAGGCTTATCCTTGGCTCGAGAAGGTTCATTTGAAGCGTATGTTTGTTAC
GGATGATGATTTGGCTCTTCTTGCTGAGTCGTTTCCTGGGTTCAAAGAGCTTACTTTGGT
CTGCTGTGAAGGTTTTGGGACTAGTGGTATTGCTATTGTTGCTAACAAATGCAGGTACTT
TGTAGATCTCTTCTCTTTGTTTGTTTGTTTGTTTGTTCTCAGAGTCTTCTAGAAATGCTT
ATCAAGTGGGACTTTGTTTTCAGGCAGCTAAAGGTCCTTGATTTGATGGAGTCAGAAGTC
ACAGATGATGAGTTGGATTGGATTTCTTGTTTTCCTGAGGGTGAAACTCATCTGGAGTCT
TTGTCTTTTGACTGTGTTGAATCCCCTATCAATTTCAAGGCATTGGAGGAGCTCGTGGTT
AGGTCACCATTCTTGAAGAAACTTAGAACGAACAGGTTTGTTTCCCTTGAAGAGCTGCAT
CGACTAATGGTTCGAGCGCCGCAGTTAACGAGTCTTGGGACGGGGTCATTTAGTCCAGAC
AATGTGCCTCAGGGAGAACAACAACCGGATTATGCAGCTGCTTTTCGTGCTTGTAAATCC
ATAGTTTGTCTCTCAGGATTCAGGGAATTTAGACCGGAATACCTCCAATATCCGGGATAT
ATGTAAATGTAAATGAGTAAAT

Last Updated on 06.12.2018 16:11