Sequences from confirmation amplicon


Confirmation sequences for line 068E03 and hit At1g51340 are shown.
Line ID 068E03
Locus At1g51340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19034732
Primers used for PCR BM09 - 8474, show primer details
   
Sequence Name 21-K015836-022-068-E03C-8409
GenBank Accession LN495446 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K015836-022-068-E03C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTATGCGATAAAGGT
TTAAGAACAGTTAAAACATGCTCTTCCCTCTAAAATTCCTTCTTCAGCTCCTAAGAAAAG
ACCAAGGTCCTGTTCCTGTCCCAATCCTGTTACAGTCACATTTACATTCTGTTATAAGAA
AGTTGGAAAAGTTTTTACATTTGTACTACAAGAAACAGAGAGTTTCACAAAGTTTTACCG
CCAGAATCCAACGGCTGCTCTAAGACTCATGTAAATGGTGAGACCAAACCAAAGTCCAAT
AAAACCATGAGTCGATGAGAGGAAGAGCAAACACAAGATGCTAACTATAGCCACCATTAC
TAATGAAGCTGCGGCGTAACCGAAATCGGATGCTCCAAAGTTAACTCCATCGAACACAAA
CGCTAAGGCATTAATTGGTTGTGTCCCTGCCACAAACTTTAGGAAACAAGAAATGATTTT
TAGCCAAATTAATGTTACGTAATAATATTGATCCTGATTAGTGGTT
   
Sequence Name 22-K015836-022-068-E03C-BM09
GenBank Accession LN495888 [GenBank]
Sequencing primer gene specific primer, BM09: 5'-TTTCCCATTAAACCACTAATCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K015836-022-068-E03C-bm09
ATATTATTACGTAACATTAATTTGGCTAAAAATCATTTCTTGTTTCCTAAAGTTTGTGGC
AGGGACACAACCAATTAATGCCTTAGCGTTTGTGTTCGATGGAGTTAACTTTGGAGCATC
CGATTTCGGTTACGCCGCAGCTTCATTAGTAATGGTGGCTATAGTTAGCATCTTGTGTTT
GCTCTTCCTCTCATCGACTCATGGTTTTATTGGACTTTGGTTTGGTCTCACCATTTACAT
GAGTCTTAGAGCAGCCGTTGGATTCTGGCGGTAAAACTTTGTGAAACTCTCTGTTTCTTG
TAGTACAAATGTAAAAACTTTTCCAACTTTCTTATAACAGAATGTAAATGTGACTGTAAC
AGGATTGGGACAGGAACAGGACCTTGGTCTTTTCTTAGGAGCTGAAGAAGGAATTTTAGA
GGGAAGAGCATGTTTTAACTGTTCTTAAACCTTTATCGCATAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT

Last Updated on 06.12.2018 16:11