Sequences from confirmation amplicon


Confirmation sequences for line 069B12 and hit At5g65160 are shown.
Line ID 069B12
Locus At5g65160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26032052
Primers used for PCR 04ke - 8474, show primer details
   
Sequence Name 58-K113393-0022-069-B12C-8409
GenBank Accession LN509760 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113393-0022-069-B12C-8409
ACTGGTTTTTATTTGAAATTCCGGTTTAATTTGATTTTATCAGATTCGACGAGGCGGTGG
AGGCGATCCAACGCGCCGGGAAGCTGGACGGGAATAACCGAGAGGTGATTATGATTTCAC
GACGGGCACAAGCGGTGACTGAAGCTCGGTTTAAAGGAAACGAGCTGTTTAAATCTGGAC
GGTTCCAAGAGGCGTGCGCCGCTTATGGTGAGGGATTAGATCACGATCCACGAAACTCTG
TTTTGCTCTGTAACCGCGCGGCTTGTCGGTCAAAATTGGGTCAATTTGATAAATCTATAG
AGGATTGCACGGCGGCTCTCTCCGTCCGGCCGGGATATGGTAAGGCTCGCCTCAGAAGAG
CCGATTGTAACGCCAAGGTAACAATAAAAACTAGTGGGGTCGATATAAAATTTTGAGAAA
ATTTGGGACTTGTTTGTAATTATTAATTAATTGCGGATGCAGATCGAAAAGTGGGAATTA
GCGGTGGGTGACTACGAGATATTGAAAAAGGAGTCGCCGGAGGATGAGCAAGTTATCAGA
GGATTATCAGAGGCTCAACAA
   
Sequence Name 77-K113394-0022-069-B12C-04ke
GenBank Accession LN513578 [GenBank]
Sequencing primer gene specific primer, 04ke: 5'-GTTGTTGAGCCTCTGATAATCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K113394-0022-069-B12C-04ke
TTTTTATATCTCGTAGTCCCCACCGCTAATTCCCACTTTTCGATCTGCATCCGCAATTAA
TTAATAATTACAAACAAGTCCCAAATTTTCTCAAAATTTTATATCGACCCCACTAGTTTT
TATTGTTACCTTGGCGTTACAATCGGCTCTTCTGAGGCGAGCCTTACCATATCCCGGCCG
GACGGAGAGAGCCGCCGTGCAATCCTCTATAGATTTATCAAATTGACCCAATTTTGACCG
ACAAGCCGCGCGGTTACAGAGCAAAACAGAGTTTCGTGGATCGTGATCTAATCCCTCACC
ATAAGCGGCGCACGCCTCTTGGAACCGTCCAGATTTAAACAGCTCGTTTCCTTTAAACCG
AGCTTCAGTCACCGCTTGTGCCCGTCGTGAAATCATAATCACCTCTCGGTTATTCCCGTC
CAGCTTCCCGGCGCGTTGGATCGCCTCCACCGCCTCGTCGAATCTGATAAAATCAAATTA
AACCGGAATTTTCAAATTAAAAACCAGTATGATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA

Last Updated on 06.12.2018 16:11