Sequences from confirmation amplicon


Confirmation sequences for line 069E09 and hit At5g64840 are shown.
Line ID 069E09
Locus At5g64840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25919518
Primers used for PCR 05ax - 3144, show primer details
   
Sequence Name 07-K106867-0022-069-E09C-3144
GenBank Accession LN489292 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106867-0022-069-E09C-3144
TAACAGCGTCAAGACGAAATCGAATCTCTTTTCTCGATCAACCCTCTCAGCAAGACTCCG
ATCGGAAACGAAACGGGAAGAGTTCCAAAAACGGAGCTTCTGGGATATCTTCAGGTGTGA
AGCTAGAGAACATAAGGAAAAGCTACAAAGGCGTGACTGTGCTTAAAGATGTGACATGGG
AAGTGAAGAGAGGTGAGAAAGTAGGGTTAGTTGGTGTGAACGGAGCTGGGAAGACGACTC
AGCTCAGGATTATCACTGGTCAAGAAGAGCCTGATTCTGGTAACGTAATCAAGGCCAAAC
CCAATATGAAAGTAGCTTTCTTGAGCCAGGAATTTGAGGTTTCGATGAGTAAGACTGTGA
GGGAAGAGTTCATGACTGCTTTCAAGGAAGAGATGGAGATTACTGAGAAGCTTGAGAAAG
TTCAGAAGGCTATTGAGGGATCTGTTGATGATTTGGATCTTATGGGAAGGTTATTAGATG
AATTTGATTTGTTGCAGA
   
Sequence Name 17-K106868-0022-069-E09C-05ax
GenBank Accession LN493871 [GenBank]
Sequencing primer gene specific primer, 05ax: 5'-TCTCTGCAACAAATCAAATTCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K106868-0022-069-E09C-05ax
GATCCCTCAATAGCCTTCTGAACTTTCTCAAGCTTCTCAGTAATCTCCATCTCTTCCTTG
AAAGCAGTCATGAACTCTTCCCTCACAGTCTTACTCATCGAAACCTCAAATTCCTGGCTC
AAGAAAGCTACTTTCATATTGGGTTTGGCCTTGATTACGTTACCAGAATCAGGCTCTTCT
TGACCAGTGATAATCCTGAGCTGAGTCGTCTTCCCAGCTCCGTTCACACCAACTAACCCT
ACTTTCTCACCTCTCTTCACTTCCCATGTCACATCTTTAAGCACAGTCACGCCTTTGTAG
CTTTTCCTTATGTTCTCTAGCTTCACACCTGAAGATATCCCAGAAGCTCCGTTTTTGGAA
CTCTTCCCGTTTCGTTTCCGATCGGAGTCTTGCTGAGAGGGTTGCTTCGAGAAAAGAGAT
TCGATTTCGTCTTGACGCTGTTTAACGGAGGTCTCGAGGCTGATGGTGGAGACTTGGGCT
CTGATGGTGGAGATATCACANCAATCCA

Last Updated on 06.12.2018 16:11