Sequences from confirmation amplicon


Confirmation sequences for line 069H03 and hit At3g23300 are shown.
Line ID 069H03
Locus At3g23300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8335465
Primers used for PCR 05hj - 8474, show primer details
   
Sequence Name 52-K112479-0022-069-H03C-05hj
GenBank Accession LN507864 [GenBank]
Sequencing primer gene specific primer, 05hj: 5'-TGTATGGTGTAAACATGGAAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K112479-0022-069-H03C-05hj
TCTTCTTTTCACTAACAGAGTTTCGTCATTAACTTCACAAGCTTGTGATATTGCATATCT
GAGAACAAATATGCTCACTAAATTATTTTTCAACGTTTCTATTAGATGACCACAAAACCA
AGGGAAGTGGATTGGCTCCATGGCCAGCTCGATTAACCTCTCCTCCTCCTCGGCTAGCTG
ACTTTGGCTATTCCACTGACATTTTTGAGAAGGATACAGTATGTTATGCCCATTATCCTT
TTTATTTGTTGCAATTGAAGCTCCAATAATATAAAGACTGTAGTCACCTTAAATCATTGC
TTGCTCATACAGGAAACTTGGAGGCAGAGAGTAGATACTTATTGGGATCTCTTGAGTCCT
AAAATTCAATCAGACACTGTAAGGAACATAATGGACATGAAAGCAAGCATGGGTTCCTTT
GCTGCTGCTCTGAAAGAAAAAGATGTTTGGGTTATGAATGTTGTTCCTGAAGACGGACCA
AACACGCTTAAACTAATATACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCA
   
Sequence Name 22-K112478-0022-069-H03C-8409
GenBank Accession LN496180 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K112478-0022-069-H03C-8409
ATTTCATGTATATTAGTTTAAGCGTGTTTGGTCCGTCTTCAGGAACAACATTCATAACCC
AAACATCTTTTTCTTTCAGAGCAGCAGCAAAGGAACCCATGCTTGCTTTCATGTCCATTA
TGTTCCTTACAGTGTCTGATTGAATTTTAGGACTCAAGAGATCCCAATAAGTATCTACTC
TCTGCCTCCAAGTTTCCTGTATGAGCAAGCAATGATTTAAGGTGACTACAGTCTTTATAT
TATTGGAGCTTCAATTGCAACAAATAAAAAGGATAATGGGCATAACATACTGTATCCTTC
TCAAAAATGTCAGTGGAATAGCCAAAGTCAGCTAGCCGAGGAGGAGGAGAGGTTAATCGA
GCTGGCCATGGAGCCAATCCACTTCCCTTGGTTTTGTGGTCATCTAATAGAAACGTTGAA
AAATAATTTAGTGAGCATATTTGTTCTCAGATATGCAATATCACAAGCTTGTGAAGTTAA
TGACGAAACTCTGTTAGTGAAAAGAAGATAGAATAATGAAAACTCACGGTCACTGTATTG
AGTAATGCATGCTTCCATGTTTACACCATAC

Last Updated on 06.12.2018 16:11