Sequences from confirmation amplicon


Confirmation sequences for line 070A03 and hit At4g32120 are shown.
Line ID 070A03
Locus At4g32120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15518267
Primers used for PCR KC40 - 8474, show primer details
   
Sequence Name 63-K035534-022-070-A03C-KC40
GenBank Accession LN510924 [GenBank]
Sequencing primer gene specific primer, KC40: 5'-CTGAATACCAAGAAACAACCCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K035534-022-070-A03C-KC40
GACTTTTGGGCAATTGTCACAGCTAGGAAATCTCAATTATGTGAACTGAAGATAGTTGAG
AATATACGAACTCTTCAGTAATCACATCTCCCGATTTCATGCACCCAATGTAAGCACCAT
CTTGACTACGACGACTTTCAAGAAGCGCAATCATCCCCTCTGTTAGACCGAAAACATTTG
TTACTCAAAGACATCGTTGATACCCTGATTGGATCAAACATAGCTTTGCAAGACAAAGAG
ATGAAAGAGGAAGTACTACCAAGATCCAAGTCAACGTTGTCATCAACTTTGACATAAAAC
TCTGCGTCCCAATTCTGAACAGCAGCACTGTAGAAGAACTTCACTTTCTTCGGTAACTCT
TCTTGGGCTTCCTCGTGATTCTCCATGTCCAAAAGAAAATGATATCAATCTTATAATGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACTAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAGATCTTTATN
TCGACGTGTCTACATTCACTCCCAATGGGG
   
Sequence Name 15-K035534-022-070-A03C-8409
GenBank Accession LN492881 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K035534-022-070-A03C-8409
GAGCCATTTACAATTGAATATATCCTGCATTATAAGATTGATATCATTTTCTTTTGGACA
TGGAGAATCACGAGGAAGCCCAAGAAGAGTTACCGAAGAAAGTGAAGTTCTTCTACAGTG
CTGCTGTTCAGAATTGGGACGCAGAGTTTTATGTCAAAGTTGATGACAACGTTGACTTGG
ATCTTGGTAGTACTTCCTCTTTCATCTCTTTGTCTTGCAAAGCTATGTTTGATCCAATCA
GGGTATCAACGATGTCTTTGAGTAACAAATGTTTTCGGTCTAACAGAGGGGATGATTGCG
CTTCTTGAAAGTCGTCGTAGTCAAGATGGTGCTTACATTGGGTGCATGAAATCTGGAGAT
GTGATTACTGAAGAGTTCGTATATTCTCAACTATCTTCAGTTCACATAATTGAGATTTCC
TAGCTGTGACAATTGCCCAAAAGTCTCATAATTAACATTCTCATTCTCATATTAAATGGG
TTGTTTCTGGGTATTCAG

Last Updated on 06.12.2018 16:11