Sequences from confirmation amplicon


Confirmation sequences for line 070C11 and hit At2g47410 are shown.
Line ID 070C11
Locus At2g47410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19450421
Primers used for PCR 09uv - 8474, show primer details
   
Sequence Name 42-K117737-0022-070-C11C-09uv
GenBank Accession LN504620 [GenBank]
Sequencing primer gene specific primer, 09uv: 5'-ATGGAAAGCAATAGCTCACTTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117737-0022-070-C11C-09uv
AGCGCAGTTTCATTGACCAATTTTGACAGCCGGTCATCGGAGCCAGTGATAACATATCTT
CCAGAACGATCAAATATAGCTGTGCAACATAAGTATAAATAGCCATACTGTTACTTTTCT
ACCCACAAAAAACATTCTATGACAATAAACGGTGCTCCTATGATGGACATACTACATTAC
AACTTCCAGATCCCCTAGTCAGAAGATGTAAACAGACTGGTATAATATCAAGGTTGCACA
TGTAACAAAACAAAGTATAGAGTTCCCACAAACCAGAGATTCAAGACAAAGGATGTATTA
TGAAGATCATACCGCAGTAGACAGCATTACGATGTCCTCTGAGCTTCTTTATATTTTGCA
TCTTTTGAACCATTGTTGATGGTTTTGCAATAGCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 10-K117736-0022-070-C11C-8409
GenBank Accession LN490846 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117736-0022-070-C11C-8409
GCCATTTACATTGAATTATCCTGCTATTGCAAAACCATCAACAATGGTTCAAAAGATGCA
AAATATAAAGAAGCTCAGAGGACATCGTAATGCTGTCTACTGCGGTATGATCTTCATAAT
ACATCCTTTGTCTTGAATCTCTGGTTTGTGGGAACTCTATACTTTGTTTTGTTACATGTG
CAACCTTGATATTATACCAGTCTGTTTACATCTTCTGACTAGGGGATCTGGAAGTTGTAA
TGTAGTATGTCCATCATAGGAGCACCGTTTATTGTCATAGAATGTTTTTTGTGGGTAGAA
AAGTAACAGTATGGCTATTTATACTTATGTTGCACAGCTATATTTGATCGTTCTGGAAGA
TATGTTATCACTGGCTCCGATGACCGGCTTGTCAAAATTTGGTCAATGGAAACTGCGCTT
TGCTTGGCTAGCTGCCGTGGGCATGAAGTGAGCTATTGCTTTCCAT

Last Updated on 06.12.2018 16:11