Sequences from confirmation amplicon


Confirmation sequences for line 070H06 and hit At2g37450 are shown.
Line ID 070H06
Locus At2g37450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15722923
Primers used for PCR CD35 - 8474, show primer details
   
Sequence Name 11-K104193-0022-070-H06C-CD35
GenBank Accession LN491091 [GenBank]
Sequencing primer gene specific primer, CD35: 5'-AGCATTAGTGATGGAGAAAGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104193-0022-070-H06C-CD35
ACTTCTTACATCACCTATAGTGTAAGATTTAGACTTACAATAAAGTAGTGCTTTTGAGAG
TAGCTTGTAGGTTATTTATTATAAAAGTCAAATTGTTTTGAACATAAAACTCATGCAGGG
GATAGTGTGCTCAGCGCTTGGTTACTACATTGGAGGAGTGGTGATGAAAACCAGAGGTCC
TGTGTTTGTAACAGCTTTCAAACCTCTTTGTATGATCGTTGTGGCGATTATGTCGAGCAT
CATCTTTGATGAGCAGATGTACCTCGGAAGGTATGTAAAAACACTGAAACACCAGCCATG
AGTTAGATAACTTTGATAGAAAGAAAAAAAAAAGAATGAAAAGATCTTGTTCTTTGGCAG
GGCTCTGGGTGCTACGGTCATATGTGTAGGTCTATACCTTGTGATATGGGGCAAAGCCAA
AGATTATGACTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCG
   
Sequence Name 27-K104194-0022-070-H06C-8409
GenBank Accession LN498363 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104194-0022-070-H06C-8409
CATAATCTTTGGCTTTGCCCCATATCACAAGGTATAGACCTACACATATGACCGTAGCAC
CAAGAGCCCTGCCAAAGAACAAGATCTTTTCATTCTTTTTTTTTTCTTTCTATCAAAGTT
ATCTAACTCATGGCTGGTGTTTCAGTGTTTTTACATACCTTCCAAGGTACATCTGCTCAT
CAAAAATGATGCTCGACATAATCGCCACAACGATCATACAAAAAGGTTTGAAAGCTGTTA
CAAACACAGGACCTCTGGTTTTCATCACCACTCCTCCAATGTAGTAACCAAGCGCTAAGC
ACACTATCCCCTGCATGAGTTTTATGTTCAAAACAATTTGACTTTTATAATAAATAACCT
ACAAGCTACTCTCAAAAGCACTACTTTATTGTAAGTCTAAATCTTACACTATAGGTGATT
GTAAAAAGTTTAGTGTCCCAACCAATGGCCCACACGCTAGGATTTCCTTTCTC

Last Updated on 06.12.2018 16:11