Sequences from confirmation amplicon


Confirmation sequences for line 071B06 and hit At1g05700 are shown.
Line ID 071B06
Locus At1g05700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1711402
Primers used for PCR KE22 - 8474, show primer details
   
Sequence Name 07-K103490-0022-071-B06C-KE22
GenBank Accession LN489235 [GenBank]
Sequencing primer gene specific primer, KE22: 5'-ATCTTAGTCTCAAACCGAACCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K103490-0022-071-B06C-KE22
CATTACGGAGTGACGTTGCTGCTGGATTCAGCCCCAAGTATCTTCAGACAAACACGTTTT
TTCTAAACCCTGAGAGCCAATCGAAAATTGCGTTTTCACTCGTTCGAACCCCAAAGTCCA
CTCTTCCGCCAATTGTTAACGCTCTAGAGATCTACGTCGCAAATAGTTTCTCGCAGTCTC
TCACTAACCAAGAGGATGGTCTGTGCTAGTATAACATGACTAGCAAAGTTCAATCTTTTT
TCTCAGTTTGATCAATGCATTCAATTGAAACTTACAGGTGACGCGGTTACGAGTCTAAAG
ACGAGTTATAAAGTGAAGAAGAACTGGCACGGAGATCCTTGTTTGCCTAATGACTACATT
TGGGAGGGCCTTAATTGCAGTTATGATAGTCTTACTCCTCCAAGAATCACATCACTGTAA
GTGTAGTCTAATTTTAATCGCTTCTTGATCAGTCGGTGGATTTTCTACATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 31-K103491-0022-071-B06C-8409
GenBank Accession LN500127 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K103491-0022-071-B06C-8409
ATTCGGACGTAGAAATCACCGACTGATCAGAAGCGATTAAAATTAGACTACACTTACAGT
GATGTGATTCTTGGAGGAGTAAGACTATCATAACTGCAATTAAGGCCCTCCCAAATGTAG
TCATTAGGCAAACAAGGATCTCCGTGCCAGTTCTTCTTCACTTTATAACTCGTCTTTAGA
CTCGTAACCGCGTCACCTGTAAGTTTCAATTGAATGCATTGATCAAACTGAGAAAAAAGA
TTGAACTTTGCTAGTCATGTTATACTAGCACAGACCATCCTCTTGGTTAGTGAGAGACTG
CGAGAAACTATTTGCGACGTAGATCTCTAGAGCGTTAACAATTGGCGGAAGAGTGGACTT
TGGGGTTCGAACGAGTGAAAACGCAATTTTCGATTGGCTCTCAGGGTTTAGAAAAAACGT
GTTTGTCTGAAGATACTTGGGGCTGAATCCAGCAGCAACTGTCACTCCGTTAATGCTGAT
GTCGAACTCTCTTGTCTGGTTCGGT
Primers used for PCR KE23 - 3144, show primer details
   
Sequence Name 45-K106973-0022-071-B06C-3144
GenBank Accession LN505542 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K106973-0022-071-B06C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAA
TACAATCGAAAAAAATTTACACTATTGGAGGCAGGAACTTATCATCAAGCGGGTTAACGG
GTCATATATCTTCCTCCTTCTCTAACCTCACAATGATTCAAGAGCTGTGAGTTTACCTAA
TTCAAGATTCTGATGATACCTATAATTTTGTATTTTGTTAAAGAGTTTTTTCTGACTTGA
ACAAGACAACTCTATTATCAGAGACTTATCAAACAATGGCTTAACCGGAGACATTCCGGA
GTTCCTCTCAAAACTGAAGTTCTTGAGGGTTTTGTAAGTTCTGGACATATCTTAACAAAT
GAGATCGATAATTATGGACTCTTAACTCTTAACAACATCTGCAGAAATCTAGAAAATAAC
ACTCTTACCGGTTCAGTTCCT
   
Sequence Name 61-K106974-0022-071-B06C-KE23
GenBank Accession LN510532 [GenBank]
Sequencing primer gene specific primer, KE23: 5'-TAATAACTCGGATGGAACTGAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K106974-0022-071-B06C-KE23
ATTCTGCAGATGTTGTAAGAGTAAGAGTCCATAATTATCGATCTCATTTGTTAAGATATG
TCCAGAACTTACAAAACCCTCAAGAACTTCAGTTTTGAGAGGAACTCCGGAATGTCTCCG
GTTAAGCCATTGTTTGATAAGTCTCTGATAATAGAGTTGTCTTGTTCAAGTCAGAAAAAA
CTCTTTAACAAAATACAAAATTATAGGTATCATCAGAATCTTGAATTAGGTAAACTCACA
GCTCTTGAATCATTGTGAGGTTAGAGAAGGAGGAAGATATATGACCCGTTAACCCGCTTG
ATGATAAGTTCCTGCCTCCAATAGTGTAAATTTTTTTCGATTGTATTCAAACACTGATAG
TTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGA
AATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCC
CTTACGTCAGTGGAGATATCAAACAAATCCA

Last Updated on 06.12.2018 16:11