Sequences from confirmation amplicon


Confirmation sequences for line 072B09 and hit At3g18820 are shown.
Line ID 072B09
Locus At3g18820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6484922
Primers used for PCR MN36 - 8474, show primer details
   
Sequence Name 20-K032414-022-072-B09C-8409
GenBank Accession LN495102 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K032414-022-072-B09C-8409
GAGCATTTACAATTGAAATGTTTGTGTTATGTCATGACTACATGAAATTGGTGTCTTTAC
TTGAGTGAAACTAATTTTGACTTCTCTGACTTGTGGTTTTGTAGGGTGGGAAAAACCTCT
TTGATGAATCAGTATGTTAATAAAAAGTTCAGCAACCAGTACAAGGCAACCATTGGAGCC
GACTTCTTGACCAAGGAAGTACAGTTTGAAGATCGACTTTTCACTTTACAGGTTCCTACT
AGTGCTTTATTAGTTAGTCACTCAATATTTTTTTTGAAGTAAGATGCTTGTGCTCAGCTC
AACCTTTCACATTTGTGTGAATTACCTTTCTTGCTTGAGCTCACTCACTTGTTCCTGTCT
TTACTTTGTCAATGTGTTTATTACAGATCTGGGATACAGCTGGACAGGAAAGGTTTCAGA
GCCTTGGTGTAGCCTTTTACCGTGGTGCTGATTGCTGTGTTCTTGTATATGATGTCAATT
CCATGAAATCATTTGAAAATCTGAACAACTGGAGGGAAGAATTTCTGATCCAGGTAACAT
ATTCA
   
Sequence Name 20-K032414-022-072-B09C-MN36
GenBank Accession LN495103 [GenBank]
Sequencing primer gene specific primer, MN36: 5'-AAAAGGCATGAATATGTTACCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K032414-022-072-B09C-MN36
GATTTTCAATGATTTCATGGAATTGACATCATATACAAGAACACAGCAATCAGCACCACG
GTAAAAGGCTACACCAAGGCTCTGAAACCTTTCCTGTCCAGCTGTATCCCAGATCTGTAA
TAAACACATTGACAAAGTAAAGACAGGAACAAGTGAGTGAGCTCAAGCAAGAAAGGTAAT
TCACACAAATGTGAAAGGTTGAGCTGAGCACAAGCATCTTACTTCAAAAAAAATATTGAG
TGACTAACTAATAAAGCACTAGTAGGAACCTGTAAAGTGAAAAGTCGATCTTCAAACTGT
ACTTCCTTGGTCAAGAAGTCGGCTCCAATGGTTGCCTTGTACTGGTTGCTGAACTTTTTA
TTAACATACTGATTCATCAAAGAGGTTTTTCCCACCCTACAAAACCACAAGTCAGAGAAG
TCAAAATTAGTTTCACTCAAGTAAAGACACCAATTTCATGTAGTCATGACATAACACAAA
CATTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTC

Last Updated on 06.12.2018 16:11