Sequences from confirmation amplicon


Confirmation sequences for line 072F02 and hit At4g02400 are shown.
Line ID 072F02
Locus At4g02400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1057723
Primers used for PCR ME27 - 8474, show primer details
   
Sequence Name 83-K027643-022-072-F02C-8409
GenBank Accession LN514251 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K027643-022-072-F02C-8409
AGCCATTTACAATTGAATATATCCTGAGGAAGTGAATCCAAACGAGAAAGCAGATGACGA
AAACCCTCGAAGCCATCAAAAACAAAGACCCAAAAAGGGTAGTAAAACCAGTAAAGGTCA
AGGCAAAAACAAGTCCAAGCTAAAGACCAAAGCTTAGCTCTCAAGATTTCTGTATTCTCA
AAACTTGTTTAGACACAAATTTTGATCCAAAAGAAAACAGTTTTGCTTTGTAATTTGTCT
TATTTATAAAAGAAACATTTTGTTTGCTTCATCAGAGCTCTTCAAATCCTTGTTCATCTC
CATCTTCTTCTACTCTTTGAAATTCTTGTCTCCATGTCTCCATCTGCAAGAATTTCACAA
TCCACTGAGTTGATAAGTAATAATGGAGACCTAGCCAGTTGTGACATTCATTACCTGATC
ATCTGAAAACTTGAGCTGTTGTGCGATAAAGAGAACCGTGTCATGGTTTACTGGTGAATG
TAATACCACACCTAAACTTGCAGCAATCTCTTCATAGCTAACTATT
   
Sequence Name 84-K027643-022-072-F02C-ME27
GenBank Accession LN514368 [GenBank]
Sequencing primer gene specific primer, ME27: 5'-TGGTTTTCGGGTGAAAATAGTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K027643-022-072-F02C-ME27
GTGTGGTATTACATTCACCAGTAAACCATGACACGGTTCTCTTTATCGCACAACAGCTCA
AGTTTTCAGATGATCAGGTAATGAATGTCACAACTGGCTAGGTCTCCATTATTACTTATC
AACTCAGTGGATTGTGAAATTCTTGCAGATGGAGACATGGAGACAAGAATTTCAAAGAGT
AGAAGAAGATGGAGATGAACAAGGATTTGAAGAGCTCTGATGAAGCAAACAAAATGTTTC
TTTTATAAATAAGACAAATTACAAAGCAAAACTGTTTTCTTTTGGATCAAAATTTGTGTC
TAAACAAGTTTTGAGAATACAGAAATCTTGAGAGCTAAGCTTTGGTCTTTAGCTTGGACT
TGTTTTTGCCTTGACCTTTACTGGTTTTACTACCCTTTTTGGGTCTTTGTTTTTGATGGC
TTCGAGGGTTTTCGTCATCTGCTTTCTCGTTTGGATTCACTTCCTCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTNTATNTCGACGTGTCTA
Primers used for PCR ME28 - 3144, show primer details
   
Sequence Name 85-K027643-022-072-F02C-3144
GenBank Accession LN514486 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K027643-022-072-F02C-3144
ATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCCTCCGNCTT
TCTTCACAACCTGCACACAAAAAGGTTATACGAGGTCAAAAGAAAGGTAACATTTTAATA
CATGGTGCAATGAGTTTAACAAATAATAAAAAGGAACCTCGGGTCGGTTGAGAGCTCCAA
CAATAGTCGCAGGATTAAACTCGGGTCCTATAGGCATGCGCATGCTGTGTTCGAAAACTT
CCTTAGATGTATATGGGAAAGGTAGAGTCGTTGTATGAAGTTTGTCAGCCTGTCAAAAAC
AGATAATGTGAGCATGAAGGGATGATTACGAATAGAGGGATCGGGATATCAAGCGAGACA
CTAACCTTTTTATCGACTTTCTCTGATATGATAACATTTCTGAGACGATAGTCTTTCCTT
GTTTTGAGATCTAGCTTTCTCTTTTTATTTGCATCTTCATGTTCTCTAACCATCCATGAA
GGTAAACCTCTCTTCTTTTGGACATTGGTCCATTGTCCCCAACCAGGAACTAGAACCGGC
TTTTCCGGTTCAGGAACTTCCTGATTTAGAACTTCTTGCTTATCCTTCTCAAATTCTTCT
ACAACGTCATCACCAGCAAAAGCACGTTG
   
Sequence Name 86-K027643-022-072-F02C-ME28
GenBank Accession LN514599 [GenBank]
Sequencing primer gene specific primer, ME28: 5'-CTCAAGCAGAGCTTATACAACGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K027643-022-072-F02C-ME28
TTGAGAAGGATAGCAAGAAGTTCTAAATCAGGAAGTTCCTGAACCGGAAAAGCCGGTTCT
AGTTCCTGGTTGGGGACAATGGACCAATGTCCAAAAGAAGAGAGGTTTACCTTCATGGAT
GGTTAGAGAACATGAAGATGCAAATAAAAAGAGAAAGCTAGATCTCAAAACAAGGAAAGA
CTATCGTCTCAGAAATGTTATCATATCAGAGAAAGTCGATAAAAAGGTTAGTGTCTCGCT
TGATATCCCGATCCCTCTATTCGTAATCATCCCTTCATGCTCACATTATCTGTTTTTGAC
AGGCTGACAAACTTCATACAACGACTCTACCTTTCCCATATACATCTAAGGAAGTTTTCG
AACACAGCATGCGCATGCCTATAGGACCCGAGTTTAATCCTGCGACTATTGTTGGAGCTC
TCAACCGACCCGAGGTTCCTTTTTATTATTTGTTAAACTCATTGCACCATGTATTAAAAT
GTTACCTTTCTTTTGACCTCGTATAACCTTTTTGTGTGCAGGTTGTGAAGAAAGCGGAGG
ATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAA
ATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC
ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAAC
GTCTTCTTTTTCCACGATGCTCCTCGTGG

Last Updated on 06.12.2018 16:11