Sequences from confirmation amplicon


Confirmation sequences for line 073A01 and hit At3g58750 are shown.
Line ID 073A01
Locus At3g58750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21725920
Primers used for PCR MS21 - 8474, show primer details
   
Sequence Name 17-K016385-022-073-A01C-8409
GenBank Accession LN493652 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K016385-022-073-A01C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCTTTCCCCTCT
TTCTTCTGTCTTTATTTGATTTAACACTTGCATTTTAAAAGGGTAGTCAGAAAAACTGTG
TTTCTTTAGTCAGTTTAAATCTGTTTCAAGCTTCAAACGTGTCTGTAACACAGGATACAT
TCTTTAATCTTCAATTGATTACAGGGGCAATAGGTCTTACAAGCCTAATCCTCGTTTGGC
TCGAGTGCTGGACATCCTCTTCATACTGCATGCTGAACATGAAATGAACTGCTCTACTGC
TGCTGCTCGGCATCTTGCCTCTAGGTACTTATCAGTTATCACTGATTAGAGATATTCATT
TAGACTTCTTTGAGTGTTTAAATCAGTATTGTGTTTCTGCAGTGGTGTTGATGTGTACAC
CGCATGTGCTGGAGCTGTTGGGGCGCTTTATGGTCCACTTCATGGTGGCGCGAACGAGGC
CGTGCTTAAGATGTTAGCAGAGATTGGGACTGCTGAAAATATTCCAGATTTCATTGAAGG
CGTGAAGAACAGGTAACTCAATGCTTAATAACTACAAGCACCAAGTACTCTGAATTGTGG
TGAGTCATGCCGGTAATGTTATATCCCTTTTCTATGTTCTCAGA
   
Sequence Name 18-K016385-022-073-A01C-MS21
GenBank Accession LN494093 [GenBank]
Sequencing primer gene specific primer, MS21: 5'-CAAAACCTGACATCTTCCTCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K016385-022-073-A01C-ms21
AAAAGGGATATAACATTACCGGCATGACTCACCACAATTCAGAGTACTTGGTGCTTGTAG
TTATTAAGCATTGAGTTACCTGTTCTTCACGCCTTCAATGAAATCTGGAATATTTTCAGC
AGTCCCAATCTCTGCTAACATCTTAAGCACGGCCTCGTTCGCGCCACCATGAAGTGGACC
ATAAAGCGCCCCAACAGCTCCAGCACATGCGGTGTACACATCAACACCACTGCAGAAACA
CAATACTGATTTAAACACTCAAAGAAGTCTAAATGAATATCTCTAATCAGTGATAACTGA
TAAGTACCTAGAGGCAAGATGCCGAGCAGCAGCAGTAGAGCAGTTCATTTCATGTTCAGC
ATGCAGTATGAAGAGGATGTCCAGCACTCGAGCCAAACGAGGATTAGGCTTGTAAGACCT
ATTGCCCCTGTAATCAATTGAAGATTAAAGAATGTATCCTGTGTTACAGACACGTTTGAA
GCTTGAAACAGATTTAAACTGACTAAAGAAACACAGTTTTTCTGACTACCCTTTTAAAAT
GCAAGTGTTAAATCAAATAAAGACAGAAGAAAGAGGGGAAAGACAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTTATTCTATTCGGAAATCT

Last Updated on 06.12.2018 16:11