Sequences from confirmation amplicon


Confirmation sequences for line 074A04 and hit At5g04130 are shown.
Line ID 074A04
Locus At5g04130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1126783
Primers used for PCR 08ra - 8474, show primer details
   
Sequence Name 73-K115895-0022-074-A04C-08ra
GenBank Accession LN512960 [GenBank]
Sequencing primer gene specific primer, 08ra: 5'-TGCAGTTCTTGTGAAGGAAGAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K115895-0022-074-A04C-08ra
TAGGAACTTCCAGCTTTTTACTCGTAAAATCCCATAGTAGCCTAGGGTCTTCCAAATCAC
TGAAGGAGATATAGAGAAAATGATAAAAGAAGAATCAATGTGAGTAAAGATTAGGACACA
CCTCAGACAAAGCATTTACTACTGATAAACCGACACCATGTAATCCACCAGACACACTGT
AGCCACTACTCGTGCCACCAAACTTGCCACCCGCATGTAAAACCTGCAGAATTAAAAAAG
ATGGCAGGTAAATCGCATAATAATAATAGTATGGTCATGAAGCTCCAACATAAACTGCAG
AACCTCGACAAATAAACTGCAGAATCTCCATATTACTATTGTGAAAAAATATAAAAGTTT
GTAGCAATACCGTAAGCACAGTCTCCAGGGAAGACTTTTTTGTCGCAATGGCTTCATTTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 43-K115894-0022-074-A04C-8409
GenBank Accession LN504956 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115894-0022-074-A04C-8409
GACAAAAAGTCTTCCCTGGAGACTGTGCTTACGGTATTGCTACAAACTTTTATATTTTTT
CACAATAGTAATATGGAGATTCTGCAGTTTATTTGTCGAGGTTCTGCAGTTTATGTTGGA
GCTTCATGACCATACTATTATTATTATGCGATTTACCTGCCATCTTTTTTAATTCTGCAG
GTTTTACATGCGGGTGGCAAGTTTGGTGGCACGAGTAGTGGCTACAGTGTGTCTGGTGGA
TTACATGGTGTCGGTTTATCAGTAGTAAATGCTTTGTCTGAGGTGTGTCCTAATCTTTAC
TCACATTGATTCTTCTTTTATCATTTTCTCTATATCTCCTTCAGTGATTTGTGAAACCCT
AGGCTACTATGGGATTTTTACGAGTAAAAAGCTGGAATGTTTCCTTATTGAATTCTAGGG
CATGTTGTTGTCAAATACTCTTCCTTCACAGNAACTGC

Last Updated on 06.12.2018 16:11