Sequences from confirmation amplicon


Confirmation sequences for line 074A12 and hit At1g14420 are shown.
Line ID 074A12
Locus At1g14420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4932445
Primers used for PCR 08de - 8474, show primer details
   
Sequence Name 20-K109778-0022-074-A12C-08de
GenBank Accession LN495252 [GenBank]
Sequencing primer gene specific primer, 08de: 5'-AAAATGCCTAGCTTATAACCCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K109778-0022-074-A12C-08de
GGCTATACCGGAAGAACTAGCCGACTGTGTGCTCGGATTCGGCCGCAGATACCACCGGAG
GAAAAGACGGACCGATATACGTCGTCAAAGACGCGTCTGACAACGATCTTATCAACCCTA
AACCAGGAACGTTAAGGCATGCAGTGACTCGAGACGGACCGCTTTGGATCATATTCGCAA
GAAGCATGATCATAAAACTGCAACAAGAACTGATGATAACGAGCGACAAAACAATCGATG
GAAGAGGAGCTAGGGTTTACATCATGGAAGGTGCTGGATTAACGTTGCAATTTGTGAATA
ATGTGATCATTCACAATATTTATGTCAAGCACATTGTTCCTGGGAACGGTGGGTTGATCA
GAGACTCTGAGGCTCACATAGGGCTTAGGACAAAAAGTGATGGTGATGGAATCAGTTTGT
TTGGAGCAACCAATATTTGGATCGACCATGTCTCAATGACAAGATGTGCAGATGGTATGA
TCGATGCTATTGACGGCTCCACTGCTGTAACTATTTCCAACAGTATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAACAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCCAAAATGGNGATGTC
   
Sequence Name 04-K109777-0022-074-A12C-8409
GenBank Accession LN487962 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K109777-0022-074-A12C-8409
ATTTACAATTGAATATATACTGTTGGAAATAGTTACAGCAGTGGAGCCCCCTTTAACATC
GATCATACCATCTGCACATCTTGTCATTGAGACATGGTCGATCCAAATATTGGTTGCTCC
AAACAAACTGATTCCATCACCATCACTTTTTGTCCTAAGCCCTATGTGAGCCTCAGAGTC
TCTGATCAACCCACCGTTCCCAGGAACAATGTGCTTGACATAAATATTGTGAATGATCAC
ATTATTCACAAATTGCAACGTTAATCCAGCACCTTCCATGATGTAAACCCTAGCTCCTCT
TCCATCGATTGTTTTGTCGCTCGTTATCATCAGTTCTTGTTGCAGTTTTATGATCATGCT
TCTTGCGAATATGATCCAAAGCGGTCCGTCTCGAGTCACTGCATGCCTTAACGTTCCTGG
TTTAGGGTTGATAA

Last Updated on 06.12.2018 16:11