Sequences from confirmation amplicon


Confirmation sequences for line 074B04 and hit At5g45650 are shown.
Line ID 074B04
Locus At5g45650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18516864
Primers used for PCR TA04 - 8474, show primer details
   
Sequence Name 14-K016273-022-074-B04C-8409
GenBank Accession LN492280 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K016273-022-074-B04C-8409
TTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTATATTATAACAGAAAACT
TAAAAGTCGTTAAATAATTTCAGAATTCTGTCATTTCATTGATTATCAGTAAAAAATAAA
ATTAAACAAAAATTTTAGTCAAAAGTTACACTTTAATTCTACACAATTATTTAAATTTTG
TTTACATAAAGAGTTTTAATGATCAATATAGAACAATATATCACATCATCGTATAGAAAA
AGAAGATTCTCGCAGCAAGCATGAGGTTCTTTGGATCTTTGCTAAAGAACCAAAGCTGAT
ATGGACGTATAGTTTACTTTTAATCTATTTCGGACCTTTAATTCATACATATATATGGTT
TGTGTGTGTGTATGTGTTTTGGCTAAAATTTGTATTTTTGGTTTTATATGTTTGGCATTT
TTGGTTTTAAATATCTGACCAATTCAAATCATAGAATTTCTATTAAAATTTGCATTTTCT
TAGAAAAAAGGGAAACATGTCTTACCGGTTACAGTGAGAAGAGTTAAAGGCAACTCCGGT
TTGGCAGATTCCTTTCCATGATTTTGGAACTGGTCCCATTCCTTTGTCATTGAAACTCTT
TGATTCTGGCCACACACCTATTATTTTAAACAAATCATTATCTCAATAATGACGGATTAA
TATAATAATGAGAT
   
Sequence Name 18-K016273-022-074-B04C-TA04
GenBank Accession LN494092 [GenBank]
Sequencing primer gene specific primer, TA04: 5'-GAGAAAAAGAGTTGCGTTATGGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K016273-022-074-B04C-ta04
TTCAAGGTTGAAGGAACTTTTTGTAGTACTAATTTATCTTTTGGAATCGTTAGGTTCATC
TCATTATTATATTAATCCGTCATTATTGAGATAATGATTTGTTTAAAATAATAGGTGTGT
GGCCAGAATCAAAGAGTTTCAATGACAAAGGAATGGGACCAGTTCCAAAATCATGGAAAG
GAATCTGCCAAACCGGAGTTGCCTTTAACTCTTCTCACTGTAACCGGTAAGACATGTTTC
CCTTTTTTCTAAGAAAATGCAAATTTTAATAGAAATTCTATGATTTGAATTGGTCAGATA
TTTAAAACCAAAAATGCCAAACATATAAAACCAAAAATACAAATTTTAGCCAAAACACAT
ACACACACACAAACCATATATATGTATGAATTAAAGGTCCGAAATAGATTAAAAGTAAAC
TATACGTCCATATCAGCTTTGGTTCTTTAGCAAAGATCCAAAGAACCTCATGCTTGCTGC
GAGAATCTTCTTTTTCTATACGATGATGTGATATATTGTTCTATATTGATCATTAAAACT
CTTTATGTAAACAAAATTTAAATAATTGTGTAGAATTAAAGTGTAACTTTTGACTAAAAT
TTTTGTTTAATTTTATTTTTTACTGATAATCAATGAAATGACAGAATTCTGAAATTATTT
AACGACTTTTAAGTTTTCTGTTATAATATAACAGGATATATTCA

Last Updated on 06.12.2018 16:11