Sequences from confirmation amplicon


Confirmation sequences for line 074E01 and hit At5g62480 are shown.
Line ID 074E01
Locus At5g62480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25088925
Primers used for PCR CD32 - 8474, show primer details
   
Sequence Name 13-K104193-0022-074-E01C-CD32
GenBank Accession LN492018 [GenBank]
Sequencing primer gene specific primer, CD32: 5'-TCCTTACAGCAAAAGGATTGAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K104193-0022-074-E01C-CD32
TATCATACCTTACCATTCGTGCAAGAAGATCTCCGAACAAGAGCCAAACCCTGCTCCGGT
ATAACCCGGTTCACAAGAAGATCCCGGTTCTTGTCCACAACGGTAAACCCATCTCTGAGT
CCTTGTTTATCATTGAATACATCGACGAAACGTGGAGTAACGGTCCACATATTTTGCCAG
AAGACCCTTATAGAAGATCTAAAGTCCGGTTTTGGGCTAACTACATCCAATTACATGTGA
GTAAATCTGATAAACCCTAACTTTTTAATTCAAGTTTTTAACTTTACTGAATTTTTATTG
TGCAGCTTTACGATTTGGTGATAAAAGTGGTGAAAAGCGAAGGAGAAGAGCAAAAGAAAG
CTCTCACAGAAGTAAAGGAGAAGCTGAGCGTTATAGAGAAAGAAGGGCTTAAGGAGATTT
TCTCTGACACTGATGGTGAACCAACTGTGACGAACGAGACCATGAGCCTCGTAGACNTTG
TGATGTGCACTTTGCTTAGCCCTTACNAGGCACACGAAGAAGTTTTGGGTCTGAAAATTA
TTGACCCAGAGTGAATAGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCG
   
Sequence Name 29-K104194-0022-074-E01C-8409
GenBank Accession LN499265 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K104194-0022-074-E01C-8409
GCTATTCACTCTGGGTCAATAATTTTCAGACCCAAAACTTCTTCGTGTGCCTTGTAAGGG
CTAAGCAAAGTGCACATCACAATGTCTACGAGGCTCATGGTCTCGTTCGTCACAGTTGGT
TCACCATCAGTGTCAGAGAAAATCTCCTTAAGCCCTTCTTTCTCTATAACGCTCAGCTTC
TCCTTTACTTCTGTGAGAGCTTTCTTTTGCTCTTCTCCTTCGCTTTTCACCACTTTTATC
ACCAAATCGTAAAGCTGCACAATAAAAATTCAGTAAAGTTAAAAACTTGAATTAAAAAGT
TAGGGTTTATCAGATTTACTCACATGTAATTGGATGTAGTTAGCCCAAAACCGGACTTTA
GATCTTCTATAAGGGTCTTCTGGCAAAATATGTGGACCGTTACTCCACGTTTCGTCGATG
TATTCAATGATAAACAAGGACTCAGAGATGGGTTTACCGTTGTGGACAAGAACCGGGATC
TTCTTGTGAACCGGGTTATACCGGAGCAGGGTTTGGCTCTGGTTCTGGAGATCTTCTTGC
ACGAATTGGTAAGGTATGGATTTAAGCCTTAGGGCTAACTCAATC

Last Updated on 06.12.2018 16:11