Sequences from confirmation amplicon


Confirmation sequences for line 074F08 and hit At3g60860 are shown.
Line ID 074F08
Locus At3g60860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22489081
Primers used for PCR GJ04 - 8474, show primer details
   
Sequence Name 02-K026969-022-074-F08C-8409
GenBank Accession LN486889 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K026969-022-074-F08C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCACAAATGA
CAAACAGAAAATTGTCAGACTACATACTTAAAGTTGCTAAGAACAGAGAACGTCTCTTAT
ATGCAGTATATGAGAGAACAGAGAGGAACGAAACTAGGAGATTCTTACGCTATCTCAACA
ATCTTTGTGAGGCTAAAAACGCGTGGATTAGAAGGAGACCGCAACTCATCCATAGAAACT
TTGCAGAGAGCTTTAACAAAGTCTATTATAGCTTCACTGTTTAATTTCTGACTCTGAGAA
AATACTTGATTCATTTCACCAACCTGCTCCAACAAGTTCAAATTTGAGACTATACTGCTC
ATCTGTTCTTGTCTCACGTTCTTAGAACCTTTTCCGCCAAGACTCATGCTGTCATATGAA
CCCCTCAAGACACCTGTAGCTGCATATTGACTT
   
Sequence Name 06-K026969-022-074-F08C-GJ04
GenBank Accession LN488659 [GenBank]
Sequencing primer gene specific primer, GJ04: 5'-AAGATGCCTGGGAGCATATACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K026969-022-074-F08C-GJ04
TGTTTCCGTTTTGAGCAACTACATCTCTTGGGAGAAGGTGCTCCTCCAGATGCCACTTTT
TTTGCTTCCAAGCAAAATGAGTCAGAGAAATCAAAGCAACCCAAACAGTATATACTTCCT
GTTCTGAAAAGGAAGGGTCCTGGGAAAAGTCAATATGCAGCTACAGGTGTCTTGAGGGGT
TCATATGACAGCATGAGTCTTGGCGGAAAAGGTTCTAAGAACGTGAGACAAGAACAGATG
AGCAGTATAGTCTCAAATTTGAACTTGTTGGAGCAGGTTGGTGAAATGAATCAAGTATTT
TCTCAGAGTCAGAAATTAAACAGTGAAGCTATAATAGACTTTGTTAAAGCTCTCTGCAAA
GTTTCTATGGATGAGTTGCGGTTCTCCTTCTAATCCACGCGTTTTTAGCCTCACAAAGAT
TGTTGAGATAGCGTAAGAATCTCCTAGTTTCGTTCCTCTCTGTTCTCTCATATACTGCAT
ATAAGAGACGTTTTCTGTT

Last Updated on 06.12.2018 16:11