Sequences from confirmation amplicon


Confirmation sequences for line 075D06 and hit At1g09080 are shown.
Line ID 075D06
Locus At1g09080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2931668
Primers used for PCR BJ79 - 8474, show primer details
   
Sequence Name 06-K104856-0022-075-D06C-BJ79
GenBank Accession LN488802 [GenBank]
Sequencing primer gene specific primer, BJ79: 5'-GCGTGATAAATAAATGGAGTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K104856-0022-075-D06C-BJ79
ACCTCACCGCAATGCAGTAGTTTTGGCATTACACTAATTCATTAATAACAAAAGAAATTA
TCCTTATGTAAATTATGACGCAAATCTTTTTATTTTATTAATCGGTACACGAAAAAAATC
TTTTAATAATTCATGCATGTGATAAAAACATTGTTAATAATTTAATTTTGATTATAATGA
TAATTGTTTTGGATAACTATACATACATACGAAGAAGTCGTAAAACCTTTGAAAGATTTT
GCGATGACGATTTAATGTCACGTGTCTGCTTGTGATTGGGTACTTTACGTGTGTAAAAAG
TAAGGAGCGCGCCAACACAAAATAACCCATTAAGCTTACGTGTCAAGAAGTGATTGGAGA
GGACACTCTACCGAGGCTAAATACGAATCATACTGAAGCACATATAAATAGACGACGAAC
TTCATACTCTTCCAAATAAACTAACAAACGAGATCGAAGAAGAGTTCTCAACAACGAAAA
ATGATTTTTATCAAGGAAAACACAGCGAAGATGACGAGGAACAAAGCTATTGCGTGTTTG
GTTTTTCTGACTGGTAAAATTCTCATAAAACACACATTTTTTGGAATTTGACGTTTCTCC
TATTTATGCTATAGTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCANAAATGTAGATT
   
Sequence Name 22-K104857-0022-075-D06C-8409
GenBank Accession LN496103 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K104857-0022-075-D06C-8409
AAGCCATTACATTGAAATATCCTGCACTATAGCATAAATAGGAGAAACGTCAAATTCCAA
AAAATGTGTGTTTTATGAGAATTTTACCAGTCAGAAAAACCAAACACGCAATAGCTTTGT
TCCTCGTCATCTTCGCTGTGTTTTCCTTGATAAAAATCATTTTTCGTTGTTGAGAACTCT
TCTTCGATCTCGTTTGTTAGTTTATTTGGAAGAGTATGAAGTTCGTCGTCTATTTATATG
TGCTTCAGTATGATTCGTATTTAGCCTCGGTAGAGTGTCCTCTCCAATCACTTCTTGACA
CGTAAGCTTAATGGGTTATTTTGTGTTGGCGCGCTCCTTACTTTTTACACACGTAAAGTA
CCCAATCACAAGCAGACACGTGACATTAAATCGTCATCGCAAAATCTTTCAAAGGTTTTA
CGACTTCTTCGTATGTATGTATAGTTATCCAAAACAATTATCATTATAATCAAAATTAAA
TTATTAACAATGTTTTTATCACATGCATGAATTATTAAAAGATTTTTTTCGTGTACCGAT
TAATAAAATAAAAAGATTTGCGTCATAATTTACATAAGGATAATTTCTTTTGTTATTAAT
GAATTAGTGTAATGCCAAAACTACTGCATATGCGGTGAGGTGTAATATTTGGAAGAACAT
GACTAATGCTTAACCACTCCTT
Primers used for PCR AM29 - 8474, show primer details
   
Sequence Name 04-K105704-0022-075-D06C-AM29
GenBank Accession LN487918 [GenBank]
Sequencing primer gene specific primer, AM29: 5'-GCGTGATAAATAAATGGAGTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K105704-0022-075-D06C-AM29
ACCTCACCGCATATGCAGTAGTTTTGGCATTACACTAATTCATTAATAACAAAAGAAATT
ATCCTTATGTAAATTATGACGCAAATCTTTTTATTTTATTAATCGGTACACGAAAAAAAT
CTTTTAATAATTCATGCATGTGATAAAAACATTGTTAATAATTTAATTTTGATTATAATG
ATAATTGTTTTGGATAACTATACATACATACGAAGAAGTCGTAAAACCTTTGAAAGATTT
TGCGATGACGATTTAATGTCACGTGTCTGCTTGTGATTGGGTACTTTACGTGTGTAAAAA
GTAAGGAGCGCGCCAACACAAAATAACCCATTAAGCTTACGTGTCAAGAAGTGATTGGAG
AGGACACTCTACCGAGGCTAAATACGAATCATACTGAAGCACATATAAATAGACGACGAA
CTTCATACTCTTCCAAATAAACTAACAAACGAGATCGAAGAAGAGTTCTCAACAACGAAN
AATGATTTTTATCAAGGAAAACACAGCGAAGATGACGAGGAACAAAGCTATTGCGTGTTT
GGTTTTTCTGACTGGTAAAATTCTCATAAAACACACATTTTTTGGAATTTGACGTTTCTC
CTATTTATGCTATAGTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCANAAATGTAGATT
   
Sequence Name 20-K105705-0022-075-D06C-8409
GenBank Accession LN495212 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K105705-0022-075-D06C-8409
GCCATTTACAATTGAATATATCCTGCACTATAGCATAAATAGGAGAAACGTCAAATTCCA
AAAAATGTGTGTTTTATGAGAATTTTACCAGTCAGAAAAACCAAACACGCAATAGCTTTG
TTCCTCGTCATCTTCGCTGTGTTTTCCTTGATAAAAATCATTTTTCGTTGTTGAGAACTC
TTCTTCGATCTCGTTTGTTAGTTTATTTGGAAGAGTATGAAGTTCGTCGTCTATTTATAT
GTGCTTCAGTATGATTCGTATTTAGCCTCGGTAGAGTGTCCTCTCCAATCACTTCTTGAC
ACGTAAGCTTAATGGGTTATTTTGTGTTGGCGCGCTCCTTACTTTTTACACACGTAAAGT
ACCCAATCACAAGCAGACACGTGACATTAAATCGTCATCGCAAAATCTTTCAAAGGTTTT
ACGACTTCTTCGTATGTATGTATAGTTATCCAAAACAATTATCATTATAATCAAAATTAA
ATTATTAACAATGTTTTTATCACATGCATGAATTATTAAAAGATTTTTTTCGTGTACCGA
TTAATAAAATAAAAAGATTTGCGTCATAATTTACATAAGGATAATTTCTTTTGTTATTAA
TGAATTAGTGTAATGCCAAAACTACTGCATATGCGGTGAGGTGTAATATTTGGAAGAACA
TGACTAATGCTTAACCACTCCAT

Last Updated on 06.12.2018 16:11