Sequences from confirmation amplicon


Confirmation sequences for line 075D09 and hit At3g02930 are shown.
Line ID 075D09
Locus At3g02930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:657256
Primers used for PCR 05aq - 8474, show primer details
   
Sequence Name 10-K106755-0022-075-D09C-8409
GenBank Accession LN490673 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K106755-0022-075-D09C-8409
TCGGACTGAGCCTGTAGTTCTTCCAGAGCCTTTGCACACTTGAAGTAGCATCTTGCTCCT
TCTTTAAAGCCTGGGTTTTCTCTTCATTCACAGTTTCAAGCTCATTCTTCAGTTTCTCTG
CTTCTTTTTCACTCTTAGATGATTCTTCTTCTGCAATACCTAGCTTCTGTTCAGATTTCT
CAAGATCAACCTTTTGACTAGCAACTGTCATCTCCAATAACTCAATTTTCTCCTTAAGAT
CAGTAATTTCAGATTCCATATCGTGCAATCTACTATTGCTTACTTCCAGCTGCTTTGTCA
CAGATACCAGAGATACCGAAGCACATTTCTCCAGCTTGTTAGCTTCTTCCAACCGTTTCT
CCAGTTCTTTCGCTTTGTTTTGCCACTCGTCAGCAAAGCCATGCGCATAAGATTCTGCCA
TTTTTGCAGCTTCTAAGTCAACATTAAGCTGCTCAATGATCATCTCTAGTTCCTTGACCT
TTGCTTCCAAACTTCTCGCATTCTCAAGGTCTCTTTTTAGATCCACTATCTCCGCCCCTA
GCTTTAAAGCAATCTCATTCTTGGAGATGATTTCCTTCTCTCGAGTTGAATCAAGCAAAG
CCTTCAAC
   
Sequence Name 26-K106756-0022-075-D09C-05aq
GenBank Accession LN497946 [GenBank]
Sequencing primer gene specific primer, 05aq: 5'-TCAGAGTTAATACGGTTGAAGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K106756-0022-075-D09C-05aq
AATCATCTCCAGAATGAGATTGCTTTAAGCTAGGGGCGGAGATAGTGGATCTAAAAAGAG
ACCTTGAGAATGCGAGAAGTTTGGAAGCAAAGGTCAAGGAACTAGAGATGATCATTGAGC
AGCTTAATGTTGACTTAGAAGCTGCAAAAATGGCAGAATCTTATGCGCATGGCTTTGCTG
ACGAGTGGCAAAACAAAGCGAAAGAACTGGAGAAACGGTTGGAAGAAGCTAACAAGCTGG
AGAAATGTGCTTCGGTATCTCTGGTATCTGTGACAAAGCAGCTGGAAGTAAGCAATAGTA
GATTGCACGATATGGAATCTGAAATTACTGATCTTAAGGAGAAAATTGAGTTATTGGAGA
TGACAGTTGCTAGTCAAAAGGTTGATCTTGAGAAATCTGAACAGAAGCTAGGTATTGCAG
AAGAAGAATCATCTAAGAGTGAAAAAGAAGCAGAGAAACTGAAGAATGAGCTTGAAACTG
TGAATGAAGAGAAAACCCAGGCTTTAAAGAAGGAGCAAGATGCTACTTCAAGTGTGCAAA
GGCTCTTGGAAGAACTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCC
Primers used for PCR 13ax - 8474, show primer details
   
Sequence Name 74-K118629-0022-075-D09C-13ax
GenBank Accession LN513152 [GenBank]
Sequencing primer gene specific primer, 13ax: 5'-TATTCATTTCTTTCCCCATTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K118629-0022-075-D09C-13ax
CCAAACCGGCTTCTCTCATCTCCAATCTACCATTGCGCTCTCAAACTGTTTCTTGGTTTG
TTCTACCGCATTGACAAGAACATCGATCTCATGTCTCGCCTCGTCAAGCATGTTCTCGTA
CTTGTTGTTCGTTGCTTTAATCACCAACTTCAGATCTTCTATCTGTGTTTCATAATTTTG
ATCACCTCGACTCAGCAATTTTTCTTTCAACTCACGTGATTCACTTGACACTTCGTGCAA
TGCTGAAGCTAAACTCTCCATTGCTTTGAGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 55-K118628-0022-075-D09C-8409
GenBank Accession LN508945 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118628-0022-075-D09C-8409
TCTCAAGCAATGGAGAGTTTAGCTTCAGCATTGCACGAAGTGTCAAGTGAATCACGTGAG
TTGAAAGAAAAATTGCTGAGTCGAGGTGATCAAAATTATGAAACACAGATAGAAGATCTG
AAGTTGGTGATTAAAGCAACGAACAACAAGTACGAGAACATGCTTGACGAGGCGAGACAT
GAGATCGATGTTCTTGTCAATGCGGTAGAACAAACCAAGAAACAGTTTGAGAGCGCAATG
GTAGATTGGGAGATGAGAGAAGCCGGTTTGGTGAATCACGTGAAGGAATTTGATGAAGAA
GTTTCTTCAATGGGGAAAGAAATGAATA

Last Updated on 06.12.2018 16:11