Sequences from confirmation amplicon


Confirmation sequences for line 075D10 and hit At5g65910 are shown.
Line ID 075D10
Locus At5g65910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26363162
Primers used for PCR CW68 - 8474, show primer details
   
Sequence Name 09-K104151-0022-075-D10C-CW68
GenBank Accession LN490152 [GenBank]
Sequencing primer gene specific primer, CW68: 5'-GCAACTAATTCCTCTGTAACTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K104151-0022-075-D10C-CW68
TCTTCTGACTCATTGTAGAAATTCGAAGCGATCTTTGTGAACTCAGACACAGGTAAGTTC
CCAGATAGTTTCGAAATCCCAGTCCTGAATCTCCCACCAATCTCAGCGAAATCGTTCTTA
ATCCCAGCGATTAGATCCTCATCTTCCTCCGGATGATCGGGAGTTTGATTTCTCTCCTGA
AGATCCGGAGATGAAGAAGGTTGCGAGAGGAACGAAGCTGTCTAAAATCAACATGGATCA
GCAATGATCCATAGTGATAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 09-K104152-0022-075-D10C-8409
GenBank Accession LN490153 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K104152-0022-075-D10C-8409
GGACTGAGCCTTTACATTGAATATATCCTATCACTATGGATCATTGCTGATCCATGTTGA
TTTTAGACAGCTTCGTTCCTCTCGCAACCTTCTTCATCTCCGGATCTTCAGGAGAGAAAT
CAAACTCCCGATCATCCGGAGGAAGATGAGGATCTAATCGCTGGGATTAAGAACGATTTC
GCTGAGATTGGTGGGAGATTCAGGACTGGGATTTCGAAACTATCTGGGAACTTACCTGTG
TCTGAGTTCACAAAGATCGCTTCGAATTTCTTACAATTGAGTTCAGAAGATGTGGATCCG
AAAGATTACGACGTAATCGGAGTTACAGAGAATTTAGTTG

Last Updated on 06.12.2018 16:11