Sequences from confirmation amplicon


Confirmation sequences for line 075E01 and hit At1g51830 are shown.
Line ID 075E01
Locus At1g51830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19244334
Primers used for PCR MS05 - 8474, show primer details
   
Sequence Name 33-K016389-022-075-E01C-8409
GenBank Accession LN500787 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K016389-022-075-E01C-8409
AATTTCCCGTAAATCTGAAGTTATGCAAATGACAAATAACTTCCAAAGAGTGCTTGGGAA
AGGAGGGTTTGGAATTGTTTATCATGGTTTGGTGAATGGTACTGAACAAGTAGCTATTAA
GATACTCTCCCATTCTTCATCTCAAGGATATAAACAATTCAAAGCTGAGGTAAATTCACA
CTTTCATCATTATCTAATTGAAACAAAAGCATCAATGATTCAAATATTTTGAAGGATATA
TATCTATAACTTCTGATTTCTGGCTGGGTATTTAGGTAGAACTTCTTCTTAGAGTTCATC
ACAAGAATTTGGTAGGCCTTGTTGGATACTGTGACGA
   
Sequence Name 34-K016389-022-075-E01C-MS05
GenBank Accession LN501209 [GenBank]
Sequencing primer gene specific primer, MS05: 5'-GTTCTCTCCTTCGTCACAGTATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K016389-022-075-E01C-ms05
AGCCTACCAAATTCTTGTGATGAACTCTAAGAAGAAGTTCTACCTAAATACCCAGCCAGA
AATCAGAAGTTATAGATATATATCCTTCAAAATATTTGAATCATTGATGCTTTTGTTTCA
ATTAGATAATGATGAAAGTGTGAATTTACCTCAGCTTTGAATTGTTTATATCCTTGAGAT
GAAGAATGGGAGAGTATCTTAATAGCTACTTGTTCAGTACCATTCACCAAACCATGATAA
ACAATTCCAAACCCTCCTTTCCCAAGCACTCTTTGGAAGTTATTTGTCATTTGCATAACT
TCAGATCTACGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11