Sequences from confirmation amplicon


Confirmation sequences for line 075E02 and hit At5g48720 are shown.
Line ID 075E02
Locus At5g48720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19759909
Primers used for PCR HV09 - 8474, show primer details
   
Sequence Name 07-K031867-022-075-E02C-8409
GenBank Accession LN489153 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K031867-022-075-E02C-8409
AGCCATTTACAATTTGAATAATGTGTATTTTTGGTGATTATAGTTATCCACTTGCCAATT
CCATGAGGAGCTACTGCAAAAAATTAACACCAAACATGATCAAACCCATAGTACATTTTA
CATGATTAGAGACTACTGTTTACTACTTTAAAGCTTTTTGAAGACTGAAGAGTCTCAACG
ACTCAAAACTAAGCAAATAGAGTAAGCACATTGCATAGTTTCAAGCTCAATCACAACATC
CCAACCTGGATTCTGACCATAGAGCACCATTTTGACAAGAGAGGGCTTACGAAACTCGGG
ATTTAGTAGAAATGATAGAGCATTGACAAGCTAAAATCCAAATCCTAAATGATTGATGAA
ACATTAAATGACCAAACATTTCTTAACTCACAATTAACATCAAATCCAACATTTCATCTT
CGCATTAAAAACACA
   
Sequence Name 08-K031867-022-075-E02C-HV09
GenBank Accession LN489619 [GenBank]
Sequencing primer gene specific primer, HV09: 5'-CTGTGTTTTTAATGCGAAGATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K031867-022-075-E02C-HV09
GATGAGTATTGTAGTTAGAATGTTTGGTCATTTATGTTTCATCAATCATTTAGGATTTGG
ATTTTAGCTTGTCAATGCTCTATCATTTCTACTAAATCCCGAGTTTCGTAAGCCCTCTCT
TGTCAAAATGGTGCTCTATGGTCAGAATCCAGGTTGGGATGTTGTGATTGAGCTTGAAAC
TATGCAATGTGCTTACTCTATTTGCTTAGTTTTGAGTCGTTGAGACTCTTCAGTCTTCAA
AAAGCTTTAAAGTAGTAAACAGTAGTCTCTAATCATGTAAAATGTACTATGGGTTTGATC
ATGTTTGGTGTTAATTTTTTGCAGTAGCTCCTCATGGAATTGGCAAGTGGATAACTATAA
TCACCAAAAATACACATTATTCAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACATTCACGTC

Last Updated on 06.12.2018 16:11