Sequences from confirmation amplicon


Confirmation sequences for line 075E09 and hit At3g17850 are shown.
Line ID 075E09
Locus At3g17850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6115576
Primers used for PCR RK50 - 8474, show primer details
   
Sequence Name 22-K016274-022-075-E09C-8409
GenBank Accession LN495891 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K016274-022-075-E09C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCATCAGGATC
TCGATTGCAGAATGTAGCATCCTCATCGGGAACTGGTATGTCCTTAGATACTAATAACAT
CTTAAAATGGTTGATGGGGGTAAGCAATGACTTTGTCTCAACCCTTTCCGTTACTTAGAT
ACTAAATGGGAATTATGAGAGAAATACGTATTGGCAGCTTGCTGTGCTGGTACTTGTGAT
AAACTTGTTCCGTGTTCTGCTTGTTATGAGTATGTTAGCTTGTATGATATCAGAGGCTTG
CTACTTTCAATATATCTGCATCTGTTTTAATAGTGGAACTTAATCACTGCCTAAACGCAT
CTTATACTGTAATGTATCCAGATCATTTGTGCCCCCTGTTAGATTTTCCGTTCTTATTTC
TCAAGTTTGCTTCTGTTGGTACTTCGCAGGTCGCTCTGAGATGTCATCCGGTCGTTCTGG
CCCTCTAAGAAATTCAGACTTTTGCACCCCAGAGGTACCCAATTATTCTCCATCACATAC
TTTTTAAAAAGTAGTTTGTGATATTTGTATTAGCTTTAGCTTTTAAATGGTCTTGGTGAC
TTGACGTTTATCTTTCTAGAATTCATATGAATGGGAAAATCCCAAAGAGTCAGAATCGCC
TCGTTATCAAGCCTTACTTCGTATGACCAGTGCTCC
   
Sequence Name 26-K016274-022-075-E09C-RK50
GenBank Accession LN497693 [GenBank]
Sequencing primer gene specific primer, RK50: 5'-TACTTCCTGGAAAAGACAGACGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K016274-022-075-E09C-rk50
ATTCTGCATACAAAAAAGATCACAAGGGGATAATAGAGATAGGAGACTTTAGAATACCTC
CACATTGTTCGACCTACGAGGCTTCCATAATGGAAAGGGACGTACACCTTTTGAGTTCAA
CTCATGAGAAAAGCTTTTGATGTCACCAGGAAACCTCTTTCGTGGAGCACTGGTCATACG
AAGTAAGGCTTGATAACGAGGCGATTCTGACTCTTTGGGATTTTCCCATTCATATGAATT
CTAGAAAGATAAACGTCAAGTCACCAAGACCATTTAAAAGCTAAAGCTAATACAAATATC
ACAAACTACTTTTTAAAAAGTATGTGATGGAGAATAATTGGGTACCTCTGGGGTGCAAAA
GTCTGAATTTCTTAGAGGGCCAGAACGACCGGATGACATCTCAGAGCGACCTGCGAAGTA
CCAACAGAAGCAAACTTGAGAAATAAGAACGGAAAATCTAACAGGGGGCACAAATGATCT
GGATACATTACAGTATAAGATGCGTTTAGGCAGTGATTAAGTTCCACTATTAAAACAGAT
GCAGATATATTGAAAGTAGCAAGCCTCTGATATCATACAAGCTAACATACTCATAACAAG
CAGAACACGGAACAAGTTTATCACAAGTACCAGCACAGCAAGCTGCCAATACGTATTTCT
CTCATAATTCCCATTTAGTATCTAAGTAACGGAAAGGGTTGAGACAAAGTCATTGCTTAC
CCCCATCAACCATTTTAAGATGTTATTAGTATCTAAGGACATACCAGTTCCCGATGAGGA
TGCTACATTCTGCAATCGAGATCCTGATGGACAGGATATATTCAATTGTAAATGGCTTCA
TGT

Last Updated on 06.12.2018 16:11