Sequences from confirmation amplicon


Confirmation sequences for line 075F01 and hit At1g53380 are shown.
Line ID 075F01
Locus At1g53380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19913359
Primers used for PCR 03wu - 8474, show primer details
   
Sequence Name 27-K118761-0022-075-F01C-03wu
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 03wu: 5'-GATGGTTCATAGACCAGCAATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118761-0022-075-F01C-03wu
CAATTATATAAAATTCTATCTTACCAACACTTCAAGTATTAAATATTATTGAATTTCAAT
CATATACGAAATCTTTTATACACCTTAAAAATTCGATAATTTCCAACATTACGTTCCTTA
TTTTATTTTTCTCCAAGTAGGCTAAAAAACATATAAAATATTTACATATTATATTAAAAA
GAGATAAATATTCAATGGACAACAGCTAAAAGATGCCTCTGTGTTCTCTGAACTAATATC
GACAAAACTCAAAAGACAGAAAAAAAAAAAACATAAACCCAACTCTGGTTCGCTACTTCA
AATCCAACGGCCCACAATTTTTCTCAATTTTCTAAACATACCCCACCATTTTCAAAAATT
ACCAAACCGCCCAAAAATCTTCACCGTCCGTTTTCGGATCTAAAATCAAACGGCGATAAT
GAAAGCGAGAGATAGTATCAAACTACAATATTTTCAATTGTAAAGGGCTTCATGTCCGGG
AAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAAAAAAAAAAAAAGTAATT
CCCATTTTTTTTTCAATTCAAAAATGTAAATTTCCCCAGCAATAAAAAAAAAA
   
Sequence Name 02-K118760-0022-075-F01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118760-0022-075-F01C-8409
TTACAATTGAATATATTGTAGTCTGATACTATCTCTCGCTTTCATGATCGCCGTTTGATC
TAAGATCCGAAAACGGACGGTGAAGATTCTTGGGCGGTTTGGTAATTTCTGAAAATGGTG
GGGTATGTTTAGAAAATTGAGAAAAATTGTGGGCCGTTGGATTTGAAGTAGCGAACTAGA
GTTGTGTTTATGTTTTTTTTTTTTCGGCCTTTTGAGTTTTGCCAATTTTAGTTCAAAAAA
CCCAAAGGCATCTTTTACCTGTTGCCCATTGAAAATTTATCCCTTTTTAAAATAAAAGGA
AAATATTTTATATGTTTTTTACCCTCCTGGGAAAAAAATAAAATAAGGAACGTATTGTTG
GAAATTATCAAATTTTTAAGGGGTATAAAAAATTTCGTATATAATTGAAATTCAATAATA
TTTAAAACTTGAAGTGTTGGTAAAAAAAAATTTTATATAATTTGCATGTACATATAACCA
TTGGACCCTTTGAAAATGTATGGCGGGCCTATAAACCA

Last Updated on 06.12.2018 16:11