Sequences from confirmation amplicon


Confirmation sequences for line 076C01 and hit At3g28520 are shown.
Line ID 076C01
Locus At3g28520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10689058
Primers used for PCR CW67 - 8474, show primer details
   
Sequence Name 10-K104151-0022-076-C01C-CW67
GenBank Accession LN490629 [GenBank]
Sequencing primer gene specific primer, CW67: 5'-AGGTGTAAACGTGGTATGGTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104151-0022-076-C01C-CW67
CATAATTCAAGGAGGGAAGTACTTGACTCTGACTTTCGAAAATCATCACAGAGATATAAT
CACAAACACTTATATTGATCATGTTTTGAGAGAAGGAAAAGAGATTGCGTTAAAGAACAG
AGAGAGGAAGCTCTACACTAACAATGACAGTAGTAGTTATTCGTCTTGGTGGGAAGGACT
ATGGAGCAACGTTCCTTTTAATCACCATGCGTCGTTCGAGACTTTGGGCATGGATCTTGA
TAAAAAAGAAGAGATTAAAAAGGATTTGATTAAGTTTACTAAAGGAAAAGATTATTATAG
GAAGGTTGCTAAGCCGTGGAAGCGCGGTTATCTCTTGTTTGGACCTCCCGGGACAGGGAA
ATCGACGATGATATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCACGATTATT
   
Sequence Name 10-K104152-0022-076-C01C-8409
GenBank Accession LN490630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104152-0022-076-C01C-8409
GCCATTTACATATCATCGTCGATTTCCCTGTCCCGGGAGGTCCAAACAAGAGATAACCGC
GCTTCCACGGCTTAGCAACCTTCCTATAATAATCTTTTCCTTTAGTAAACTTAATCAAAT
CCTTTTTAATCTCTTCTTTTTTATCAAGATCCATGCCCAAAGTCTCGAACGACGCATGGT
GATTAAAAGGAACGTTGCTCCATAGTCCTTCCCACCAAGACGAATAACTACTACTGTCAT
TGTTAGTGTAGAGCTTCCTCTCTCTGTTCTTTAACGCAATCTCTTTTCCTTCTCTCAAAA
CATGATCAATATAAGTGTTTGTGATTATATCTCTGTGATGATTTTCGAAAGTCAGAGTCA
AGTACCTTCCCTCCTTCGAATTATGTTTATCCTCTTTATCTACCACGGTGGAAGACCATA
C

Last Updated on 06.12.2018 16:11