Sequences from confirmation amplicon


Confirmation sequences for line 076F12 and hit At5g59260 are shown.
Line ID 076F12
Locus At5g59260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23909335
Primers used for PCR KE19 - 8474, show primer details
   
Sequence Name 38-K103676-0022-076-F12C-KE19
GenBank Accession LN502949 [GenBank]
Sequencing primer gene specific primer, KE19: 5'-TCTCTGCCACATACTGTTTCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K103676-0022-076-F12C-KE19
CTCTTACCGCGATTTGTGTACCAGAAGGAAGTATTCCTTTATAGACTTTTCCAAAACCTC
CTGCTCCAAGTAACTGATTCTCCCTGAAACCTTTGGTTGCTTTGTAAAGGATCCTAAAGG
AGTACCTTTGAGGGCTATATTCCTTTTCCCATTGTTCAAGCACCTCTGCATACTTCTTTT
TCTTGTAAAGATACACAATTCCTCCCAGAGTTAGAAAAGCAATTGTTGATATGGTAGCGC
CTAGAACCTCTTTTAATGGAAACCTTTTAGGCGATGGTGGTGGATTCGAAAGTCTTGAAA
TGTCAAGGCTTTCCGCTTTTCCGCCTTGCTTGAAACTCCATCCAAGGATATACTGGTTAC
TTTTGATTGAGCCTGTAGAACCAGAAAAACCTACATACATCGTCTCAGTGAAAATTTCTG
AAAGGTTGATGGATCTTGACAAGAGTGGCACTTTTGGTTTAATGAAGCTTGAAAAGTAAG
GCGAATCGGGCTTTGGAGTTTGTACTGGAGCTAACGTTACGTTGAGTACTTTCTCTATTC
CATCATAATCTATCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCA
   
Sequence Name 54-K103677-0022-076-F12C-8409
GenBank Accession LN508451 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K103677-0022-076-F12C-8409
GCCATTTACAATTGGATAGATTATGATGGAATAGAGAAAGTACTCAACGTAACGTTAGCT
CCAGTACAAACTCCAAAGCCCGATTCGCCTTACTTTTCAAGCTTCATTAAACCAAAAGTG
CCACTCTTGTCAAGATCCATCAACCTTTCAGAAATTTTCACTGAGACGATGTATGTAGGT
TTTTCTGGTTCTACAGGCTCAATCAAAAGTAACCAGTATATCCTTGGATGGAGTTTCAAG
CAAGGCGGAAAAGCGGAAAGCCTTGACATTTCAAGACTTTCGAATCCACCACCATCGCCT
AAAAGGTTTCCATTAAAAGAGGTTCTAGGCGCTACCATATCAACAATTGCTTTTCTAACT
CTGGGAGGAATTGTGTATCTTTACAAGAAAAAGAAGTATGCAGAGGTGCTTGAACAATGG
GAAAAGGAATATAGCCCTCAAAGGTACTCCTTTAGGATCCTTTACAAAGCAACCAAAGGT
TTCAGGGAGAATCAGTTACTTGGAGCAGGAGGTTTTGGAAAAGTCTATAAAGGAATACTT
CCTTCTGGTACACAAATCGCGGTTAAGAGAGTCTACCATGATGCAGAGCAAGGAATGAAA
CAGTATGTGGGCAG
Primers used for PCR 57wz - 3144, show primer details
   
Sequence Name 32-K118781-0022-076-F12C-35St
GenBank Accession LN500773 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118781-0022-076-F12C-35St
CCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGAATACAAACAGAATA
CTTTTACCACTAGCAAGCGGCAGACTTATATTCTTTCCTTTTCTAGCATTAAAGTAACTA
GCGTCCGCAGATTCAACTGAAACTATACTGTTAATATCAATACCCACATCGTTACCACCT
TTGTCAAATGACTCCGAGTTTTGATTAGTGTCAAGTTCTACAGCAAAGATATGGGTTGCA
GTCTTGTTATCATTTGCTCTATTGAAAATACCTAAATTTGAATTTGCACTGCCGTTAGAC
CTGAGATCTTTGGTTGGAGATACCACAAATGCCATTCCGTGACCGTAGGTAGATTTTTGC
AGCGGGAAGATTGCAAAGACAAATTCTGTTGAGAAGGAAAATGAACTGAGTGAAGACGCT
GTGAACTTGATGGGAATGTTGTAGAAAGCGTGACCGGTGCCCGTGTTTGTATTATTTGTT
AGATGCAATGGACCATGGTTTGTATTTGCCATTCCATCGAGTTCCAGATCTGCATTCCTA
AAGTCATAATACACAAAATGATCTCCCTCTTGAGCGAACACCAAAAAGGTTACATGGATA
CCAATTATCAACCAGAAAACCACTGATCCTAGAACTCCAGCCATCAGGAACAACGATGAT
CTTCTCTCCAACCTTAGCTTGTAAATCTCAGATTGTTCTGCACCTTTNTTTCTTAACCAT
GAGAATTGTGTTTTCTGACTTCAGAAAACCCTGAGTTGACATTTGGAAGACACTATAANA
GAAAAGCCAAGAAAGATTCTTTCCTTTTGGATTGGGTATAGATCAATGTGCCTCTGTCAT
   
Sequence Name 72-K118782-0022-076-F12C-57wz
GenBank Accession LN512809 [GenBank]
Sequencing primer gene specific primer, 57wz: 5'-CCAAAGTAATTGACCCACAAAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K118782-0022-076-F12C-57wz
TGATCTATTACCAATCCAAAGGAAAGAATCTTTCTTGGCTTTTCTTTTATAGTGTCTTCC
AAATGTCAACTCAGGGTTTTCTGAAGTCAGAAAACACAATTCTCATGGTTAAGAAAAAAA
GGTGCAGAACAATCTGAGATTTACAAGCTAAGGTTGGAGAGAAGATCATCGTTGTTCCTG
ATGGCTGGAGTTCTAGGATCAGTGGTTTTCTGGTTGATAATTGGTATCCATGTAACCTTT
TTGGTGTTCGCTCAAGAGGGAGATCATTTTGTGTATTATGACTTTAGGAATGCAGATCTG
GAACTCGATGGAATGGCAAATACAAACCATGGTCCATTGCATCTAACAAATAATACAAAC
ACGGGCACCGGTCACGCTTTCTACAACATTCCCATCAAGTTCACAGCGTCTTCACTCAGT
TCATTTTCCTTCTCAACAGAATTTGTCTTTGCAATCTTCCCGCTGCAAAAATCTACCTAC
GGTCACGGAATGGCATTTGTGGTATCTCCAACCAAAGATCTCAGGTCTAACGGCAGTGCA
AATTCAAATTTAGGTATTTTCAATAGAGCAAATGATAACAAGACTGCAACCCATATCTTT
GCTGTAGAACTTGACACTAATCAAAACTCGGAGTCATTTGACAAAGGTGGTAACGATGTG
GGTATTGATATTAACAGTATAGTTTCAGTTGAATCTGCGGACGCTAGTTACTTTAATGCT
AGAAAAGGAAAGAATATAAGTCTGCCGCTTGCTAGTGGTAAAAGTATTCTGTTTGTATTC
ACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCT
CAAATGAAATGAACTTCCCTATATAGAGGAAGGTCTTGCGAAGATAGTGGGATGTGCGTC
ATCCC

Last Updated on 06.12.2018 16:11