Sequences from confirmation amplicon


Confirmation sequences for line 077H01 and hit At1g05810 are shown.
Line ID 077H01
Locus At1g05810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1748323
Primers used for PCR NE23 - 8474, show primer details
   
Sequence Name 56-K102433-0022-077-H01C-8409
GenBank Accession LN509060 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K102433-0022-077-H01C-8409
CATTTACATTGAATAATCCTGCACAATATAATTATGAAAAAGAGGACATACTGCGCATTA
AGTGCACCTTCTTTAATGTCGTTACCTTTTTCTTTTTCTATATTTTCCTCCAAATTATCA
GTTTTAATTTACGACTTTTCCCTGATTTGTGTTTTTGGTTAGTTATTTTAAAATCCACCA
CGTTGGTGGACTTCTTGGTAACGTAGGAGGGAGTTATATTTTTTTAACTTACAAAACCAA
AGTGTATGTATAACTAAAGAAATTTCATCTCTAATAAAGAATAAATCTTTCTCTGTAACG
TGCATACACGCTAGTTTTGTTAATAATAGGAAAGTTAATGCTACTAATAGTATTCCGGTT
CATACATTTTATGCCAATTTTCATATCAACCTATATCTATTTGCATGTTTCTAACTACTA
CTAAATATATCATAATTCATTATATGATCAACGAGATTCACTTTGGTCTTTGGTACG
   
Sequence Name 56-K102434-0022-077-H01C-NE23
GenBank Accession LN509061 [GenBank]
Sequencing primer gene specific primer, NE23: 5'-ACGTACCAAAGACCAAAGTGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K102434-0022-077-H01C-NS23
ATGATATATTTAGTAGTAGTTAGAAACATGCAAATAGATATAGGTTGATATGAAAATTGG
CATAAAATGTATGAACCGGAATACTATTAGTAGCATTAACTTTCCTATTATTAACAAAAC
TAGCGTGTATGCACGTTACAGAGAAAGATTTATTCTTTATTAGAGATGAAATTTCTTTAG
TTATACATACACTTTGGTTTTGTAAGTTAAAAAAATATAACTCCCTCCTACGTTACCAAG
AAGTCCACCAACGTGGTGGATTTTAAAATAACTAACCAAAAACACAAATCAGGGAAAAGT
CGTAAATTAAAACTGATAATTTGGAGGAAAATATAGAAAAAGAAAAAGGTAACGACATTA
AAGAAGGTGCACTTAATGCGCAGTATGTCCTCTTTTTCATAATTATATTGTGCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTCA

Last Updated on 06.12.2018 16:11