Sequences from confirmation amplicon


Confirmation sequences for line 078D02 and hit At5g62680 are shown.
Line ID 078D02
Locus At5g62680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25166441
Primers used for PCR RU47 - 8474, show primer details
   
Sequence Name 03-K024722-022-078-D02C-8409
GenBank Accession LN487320 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K024722-022-078-D02C-8409
CCGGACATGAAGCCATTTACAATTGAGCTTGGAGTTTGCGTATTTTGGTGGGTAGTAATT
GTAAAGGTTAAGCCAAGGCTGTTTCGCGGGCTTTAATCCGCGTTTCTTGATCGCAACCGC
TATAACTTGAGCTATACCGGCCAATGGACTACCCGAGGCTTTGATCTTCACATACAACTT
ATCGCCCGCAAAGAAAATCAAGCAGGCCAAAAACATTAGAACGGCAGGGATGGTTAGACC
GATCGTCCAACTGACATTAGACTGAACGTAGACGACTAGAGTCAGCGACAGGATCTGCGC
GAAAGTGAAGGTAAAGAAATACCAATTAAAGAAACTGTCAATCCCTCTTTTCCCTGATTC
GCTTTTCGGGTTAAACTGATCAGCCCCAAAAGCTAAGTTACACGGTCTAATACCGCCCGC
TCCCACTACAAGGAACCCGAGACCCATCAGTAGAAACGCTATCTGGCCTCCACTTGGCCC
GTTACATATGCTATCCGCCGCTGTTCCACATGCAGCTGGATGCAGTTGTGGCACTGCTGC
AGTCAATAGTATCACAAATGATCCCTAC
   
Sequence Name 04-K024722-022-078-D02C-RU47
GenBank Accession LN487759 [GenBank]
Sequencing primer gene specific primer, RU47: 5'-GTAAATTCGGTTTTGTAGGGATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K024722-022-078-D02C-RU47
TGATACTATTGACTGCAGCAGTGCCACAACTGCATCCAGCTGCATGTGGAACAGCGGCGG
ATAGCATATGTAACGGGCCAAGTGGAGGCCAGATAGCGTTTCTACTGATGGGTCTCGGGT
TCCTTGTAGTGGGAGCGGGCGGTATTAGACCGTGTAACTTAGCTTTTGGGGCTGATCAGT
TTAACCCGAAAAGCGAATCAGGGAAAAGAGGGATTGACAGTTTCTTTAATTGGTATTTCT
TTACCTTCACTTTCGCGCAGATCCTGTCGCTGACTCTAGTCGTCTACGTTCAGTCTAATG
TCAGTTGGACGATCGGTCTAACCATCCCTGCCGTTCTAATGTTTTTGGCCTGCTTGATTT
TCTTTGCGGGCGATAAGTTGTATGTGAAGATCAAAGCCTCGGGTAGTCCATTGGCCGGTA
TAGCTCAAGTTATAGCGGTTGCGATCAAGAAACGCGGATTAAAGCCCGCGAAACAGCCTT
GGCTTAACCTTTACAATTACTACCCACCAAAATACGCAAACTCCAAGCTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11