Sequences from confirmation amplicon


Confirmation sequences for line 078G11 and hit At4g37490 are shown.
Line ID 078G11
Locus At4g37490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17622579
Primers used for PCR AS12 - 8474, show primer details
   
Sequence Name 43-K018752-022-078-G11C-AS12
GenBank Accession LN504679 [GenBank]
Sequencing primer gene specific primer, AS12: 5'-AAATTATCATTTGGATTGGCAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K018752-022-078-G11C-AS12
GATTACATGGCATCTCAGCCTGATATTAATGAAAAGATGAGACTGATCCTGGTGGAGTGG
TTGATTGATGTGCATGTCCGATTCGAGCTAAACCCGGAAACATTTTACCTCACTGTTAAC
ATTCTGGATCGGTTCTTGTCGGTTAAGCCAGTGCCTCGAAAAGAACTGCAGCT
   
Sequence Name 47-K018752-022-078-G11C-8409
GenBank Accession LN506045 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K018752-022-078-G11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAACTGAATGTTCAATATGATGAAATGTATAAC
CATGGAAGAAACTAAAAAAACGCTCTCTTACCTTTTCGTCTGCAATGGAAGCTTTGATGA
AACGAGCTAGGAAGACATAATGAGTCGGAACTGTCAAGTACCACTCAAGTGTAGACAGTA
TTGTCTTCTCCATCACCAGAATCTGTTTGTGACTGTATGCATGGTCTGCAATATCAACTA
GATCCTCCACCTGCAAAAAAATTATCTTTTCATTTAGCCGAAGACAAAAAAGATTCAAAA
CCAAAAAATTCAAAAGTTCTCTGTTTTTATCACCTGGGGGGGCCAAATTTCTTCATACTT
GGCCGACTTGAAAAAAGCACTGAAACCAACAAGCTGCAGTTCT

Last Updated on 06.12.2018 16:11