Sequences from confirmation amplicon


Confirmation sequences for line 078H09 and hit At4g32160 are shown.
Line ID 078H09
Locus At4g32160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15532669
Primers used for PCR 06sf - 8474, show primer details
   
Sequence Name 40-K116491-0022-078-H09C-06sf
GenBank Accession LN503835 [GenBank]
Sequencing primer gene specific primer, 06sf: 5'-TACTAGAGAACACAGTTGCGGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K116491-0022-078-H09C-06sf
GACCGGCTTAAGAGTACAACTTAAATTTGTCAATGGATGGAAACGGTAACTTTGTCGACG
ACTCAACTACAATATCAGACGTTTTGCGTTTACTGTCAATATCTGATGATCAAATTGAGG
AGGTATTGCTGCATCACTGCTTTATTGCCATTTAAAATCAGTTTCCTCTAATAAGAGTTA
CTTTTCACGATATGTCCATCAGGATGAAGTTATTTATTACTTATTAGTGTAGGATAGTTA
GGTTTCCGCTGCACTAATGAAAACTGTAACGTTGTGCATTTTACAAGTTTTAACGATTTA
GACCATTTTCAGGCTCAGCTACTTTCTGGGTTTGACGAAAATGCTGCTGCTGATGGATCT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
ATACGCACGATTATTAT
   
Sequence Name 08-K116490-0022-078-H09C-8409
GenBank Accession LN489891 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116490-0022-078-H09C-8409
ATTTACATTGAAAGATCCATCAGCAGCAGCATTTTCGTCAAACCCAGAAAGTAGCTGAGC
CTGAAAATGGTCTAAATCGTTAAAACTTGTAAAATGCACAACGTTACAGTTTTCATTAGT
GCAGCGGAAACCTAACTATCCTACACTAATAAGTAATAAATAACTTCATCCTGATGGACA
TATCGTGAAAAGTAACTCTTATTAGAGGAAACTGATTTTAAATGGCAATAAAGCAGTGAT
GCAGCAATACCTCCTCAATTTGATCATCAGATATTGACAGTAAACGCAAAACGTCTGATA
TTGTAGTTGAGTCGTCGACAAAGTTACCGTTTCCATCCATTGACAAATTTAAGTTGTACT
CTTTAAGCCGGTCGTGGAGAATTCTGCAGTCACTAAGCAGCTTCTTTCTAGCCGCAACTG
TGTTCTCTAGTA

Last Updated on 06.12.2018 16:11